食品科学

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L-半胱氨酸对乙醛消除及A549细胞内环境抗氧化作用

张晓红,罗 成*   

  1. 天津科技大学食品科学与生物技术学院,天津 300457
  • 出版日期:2013-07-15 发布日期:2013-06-28
  • 基金资助:

    天津市2010年千人计划启动经费项目(91111114)

Effect of L-Cysteine on Elimination of Acetaldehyde and Intracellular Redox Status in Lung Cancer Line A549

ZHANG Xiao-hong, LUO Cheng*   

  1. College of Food Science and Biological Technology, Tianjin University of Science and Technology, Tianjin 300457, China
  • Online:2013-07-15 Published:2013-06-28

摘要:

为了研究L-半胱氨酸对乙醛的消除作用及对A549细胞内环境抗氧化的影响,在分析乙醛对非小细胞肺癌 A549细胞损伤影响的基础上,重点研究了L-半胱氨酸对乙醛损伤非小细胞肺癌A549细胞增殖、乳酸脱氢酶(LDH) 释放、超氧化物歧化酶(SOD)活性和一氧化氮(NO)含量的影响。结果显示:乙醛对A549细胞具有明显的损伤作 用,且呈剂量依赖性;L-半胱氨酸对乙醛损伤的A549细胞具有保护作用并增强其抗氧化性。在L-半胱氨酸浓度为 0~160μmol/L范围内,L-半胱氨酸处理对乙醛损伤的A549细胞的LDH活力和NO含量与L-半胱氨酸实验剂量呈负相 关,SOD活性与L-半胱氨酸实验剂量呈正相关。

关键词: 乙醛, L-半胱氨酸, 抗氧化, A549细胞

Abstract:

Local acetaldehyde exposure from alcohol drinking and smoking is a major factor behind different diseases including
cancers. Thus, decreasing the production or eliminating acetaldehyde locally might offer a preventive strategy against acetaldehydeinduced
cancers. In this study, the influence of acetaldehyde on the cell proliferation and intracellular antioxidants of non small cell
lung cancer A549 cells including the leakage of lactate dehydrogenase (LDH), superoxide dismutases (SOD) activity and nitric
oxide (NO) content was investigated. The protection effect of L-cysteine on A549 cells was also studied. Our results showed that
the cytotoxicity of acetaldehyde on A549 cell growth was dose-dependent. This acetaldehyde-induced cytotoxicity was inhibited
by L-cysteine within the concentration range of 0—160 μmol/L. In addition, the antioxidant status was enhanced. Increasing
L-cysteine concentration led to a reduction in LDH activity and NO content but an increase in SOD activity.

Key words: acetaldehyde, L-cysteine, antioxidant, A549 cell

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