食品科学

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影响β-胡萝卜素降解菌酶活性因素的研究

王树林1,朱明明2,李 婧3,左伟平1   

  1. 1.青海大学农牧学院,青海 西宁 810016;2.西北农林科技大学食品科学与工程学院,陕西 杨凌 712100;
    3.河南农业大学食品科学技术学院,河南 郑州 450002
  • 出版日期:2013-07-15 发布日期:2013-06-28
  • 通讯作者: 王树林
  • 基金资助:

    国家自然科学基金项目(31060225)

Optimization of Factors Affecting Microbial Production of β-Carotene Degradating Enzymes

WANG Shu-lin1,ZHU Ming-ming2,LI Jing3,ZUO Wei-ping1   

  1. 1. College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China;
    2. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;
    3. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China
  • Online:2013-07-15 Published:2013-06-28
  • Contact: WANG Shu-lin

摘要:

采用单因素试验和响应面分析法对影响β-胡萝卜素降解菌粗酶液酶活的主要因素进行优化,建立主要试验 因素与响应值之间的函数关系,并筛选得到影响酶活性的主要培养基组成及最优培养条件,主要培养基组成为:蔗 糖30g/L、酵母浸粉4g/L;培养条件:接种量7%、培养温度36℃、pH7、培养时间36h,此条件下β-胡萝卜素降解菌 酶活力达到750.72U/L。

关键词: &beta, -胡萝卜素降解菌, 响应面分析法, 优化

Abstract:

Response surface methodology (RSM) was employed to optimize medium components and culture conditions
for the production of enzymes capable of β-carotene degradation. A response surface equation was developed indicating the
effects of the main variables identified by one-factor-at-a-time design on the β-carotene degradation activity of crude enzyme
solution. Results showed that the highest β-carotene dagradation activity of 750.72 U/L was achieved under the conditions:
36 h of culture at 36 ℃ with an inoculum concentration of 7% in a liquid nutrient medium containing sucrose 30 g/L and
yeast extract powder 4 g/L at pH 7.

Key words: microbial &beta, -carotene degradation;response surface methodology (RSM);optimization

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