食品科学
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卫晓怡1,白 晨1,*,崔琳琳1,陆 红2, 蒲立柠1
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上海商学院启明星项目(QMX2012-11);2012年度上海商学院校级教学团队建设项目; 上海市教育委员会科研创新项目(10YZ212)
WEI Xiao-yi1,BAI Chen1,*,CUI Lin-lin1,LU Hong2,PU Li-ning1
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摘要:
通过在KKAy小鼠膳食中添加花色苷Cy-3-g(1g/kg膳食)干预12周,分析Cy-3-g对小鼠血糖、血清胰岛素含 量的作用,并进一步检测肝脏、肌肉、内脏脂肪组织PTEN mRNA、蛋白以及磷酸化Akt蛋白的表达。结果表明: Cy-3-g膳食干预可以改善KKAy小鼠胰岛素敏感性,抑制肝脏、肌肉、内脏脂肪组织PTEN表达而增强Akt磷酸化, 提示富含Cy-3-g的花色苷提取物可能是通过抑制PTEN表达而激活PI3K/Akt通路,从而改善小鼠胰岛素抵抗。
关键词: 矢车菊素-3-葡萄糖苷, 胰岛素抵抗, PTEN, KKAy小鼠
Abstract:
Cyanidin-3-O-β-glucoside (Cy-3-g)-rich foods have been reported to ameliorate the onset of insulin resistance, but the underlying mechanism has remained unclear. PTEN (phosphatase and tensin homologue deleted on chromosome 10) plays a negative role in insulin signaling-PI3K (phosphatidylinositol 3-kinase)/Akt (protein kinase B), and its inhibition improves insulin sensitivity. In order to gain insight into the effect of Cy-3-g anthocyanin on insulin sensitivity and the role of PTEN, KKAy mice were fed AIN-93G diet supplemented with or without Cy-3-g (1 g/kg diet) for 12 weeks. Our results demonstrated that Cy-3-g ameliorated insulin sensitivity, and inhibited the PTEN expression in liver, muscle and viscera adipose tissues following the activation of Akt (Ser473) in mice. These findings provided evidence for the role of PTEN in PI3K/Akt (Ser473) pathway, thus suggesting that the possible mechanism may be the contribution of Cy-3-g to insulin signaling.
Key words: cyanidin-3-O-&beta, -glucoside;insulin resistance;PTEN;KKAy mice
中图分类号:
TQ936.16
卫晓怡1,白 晨1,*,崔琳琳1,陆 红2, 蒲立柠1. 矢车菊素-3-葡萄糖苷抑制KKAy糖尿病小鼠PTEN表达[J]. 食品科学, doi: 10.7506/spkx1002-6630-201313059.
WEI Xiao-yi1,BAI Chen1,*,CUI Lin-lin1,LU Hong2,PU Li-ning1. Cyanidin 3-Glucoside Ameliorates Insulin Sensitivity through Down-regulation of PTEN in Diabetic KKAy Mice[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201313059.
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https://www.spkx.net.cn/CN/Y2013/V34/I13/280