食品科学 ›› 2014, Vol. 35 ›› Issue (4): 131-136.doi: 10.7506/spkx1002-6630-201404027

• 分析检测 • 上一篇    下一篇

液相色谱-质谱检测3 种不同属新鲜黄芩中8种成分

董 喆1,黄湘鹭1,曹 进1,*,张庆生1,肖盛元2,Ian B. Cole3,Susan J. Murch3   

  1. 1.中国食品药品检定研究院食品化妆品检定所,北京 100050;2.北京理工大学生命科学院,北京 100081;
    3.卑诗大学奥根分校科学与艺术学院化学系,大不列颠哥伦比亚 基洛纳 V1V1V7
  • 收稿日期:2013-04-25 修回日期:2014-01-22 出版日期:2014-02-25 发布日期:2014-03-17
  • 通讯作者: 曹进 E-mail:cao1208@gmail.com
  • 基金资助:

    科技部国际合作课题(2011DFA31440);“十二五”国家科技支撑计划项目(2012BAK08B02)

Determination of 8 Components in Fresh Samples of 3 Scutellaria Species Using On-Line Liquid Chromatography-Tandem Mass Spectrometry

DONG Zhe1, HUANG Xiang-lu1, CAO Jin1,*, ZHANG Qing-sheng1, XIAO Sheng-yuan2, Ian B. Cole3, Susan J. Murch3   

  1. 1. Institute of Food and Cosmetics Control, National Institutes of Food and Drug Control, Beijing 100050, China;
    2. School of Life Science, Beijing Institutes of Technology, Beijing 100081, China;
    3. Chemistry, I.K. Barber School of Arts and Sciences, University of British Columbia Okanagan, Kelowna V1V1V7, Canada
  • Received:2013-04-25 Revised:2014-01-22 Online:2014-02-25 Published:2014-03-17
  • Contact: Jin Cao E-mail:cao1208@gmail.com

摘要:

目的:利用在线的方式,建立对3 个属的新鲜黄芩样品中8 种成分(褪黑素、5-羟色胺、吲哚-3-乙酸、金丝桃素、黄芩苷、黄芩素、汉黄芩苷、野黄芩苷)的液相色谱-质谱联用测定方法。方法:植物样品的匀浆与适量填料混合后,被填入相应的样品仓,通过杂质去除,目标物质富集洗脱等步骤,用于成分测定。结果:测定黄芩样品中8 种成分,方法的检出限及定量限条件的回收率均大于90%,重复性及稳定性相对标准偏差不大于3.1%。结论:可以用于对黄芩原料中功效成分的评价。

关键词: 液相色谱-质谱法, 中国黄芩, 北美黄芩, 南美黄芩

Abstract:

Objective: An on-line liquid chromatography-tandem mass spectrometry method was developed to determine 8
components in fresh Scutellaria samples from three germplasm sources. Methods: Mixtures of homogenated tissue samples and
appropriate amounts of packing material were packed in sample chamber. After removing matrix impurities and enriching the
target analytes, 8 components in fresh Scutellaria samples were determined. Results: The recoveries were greater than 90% at the
limits of detection and quantification. Meanwhile, the RSDs of repeatability and stability were less than 3.1%. Conclusion: The
method developed can be used for the determination of active components in Scutellaria samples.

Key words: liquid chromatography-mass spectrometry (LC-MS), Scutellaria baicalensis Georgi, Scutellaria lateriflora L., Scutellaria racemosa Pers.

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