食品科学

• 工艺技术 • 上一篇    下一篇

响应面法优化黄秋葵果胶微波辅助提取工艺

李加兴,覃敏杰,吴 越,张 娟,周炎辉   

  1. 1.吉首大学食品科学研究所,湖南 吉首 416000;2.湖南奇异生物科技有限公司,湖南 长沙 410008
  • 出版日期:2013-07-25 发布日期:2013-08-02

Optimization of Microwave-Assisted Extraction Conditions for Pectin from Okra by Response Surface Methodology

LI Jia-xing,QIN Min-jie,WU Yue,ZHANG Juan,ZHOU Yan-hui   

  1. 1. Institute of Food Science, Jishou University, Jishou 416000, China;
    2. Hunan Amazing Grace Biotechnology Co. Ltd., Changsha 410008, China
  • Online:2013-07-25 Published:2013-08-02

摘要:

以黄秋葵干果为原料,采用响应面法对其果胶微波辅助酸提醇沉工艺进行优化。首先采用单因素试验探讨了微波功率、微波时间、浸提pH值、料液比、浸提温度及浸提时间对提取黄秋葵果胶得率的影响,然后再应用Box-Behnken方法建立数学模型,研究黄秋葵果胶的最佳提取工艺条件。结果表明,在微波时间60s、料液比1:20(g/mL)的条件下,采用微波辅助酸提醇沉法提取黄秋葵果胶的最佳工艺参数为微波功率590W、pH2.9、浸提温度74℃、浸提时间30min,此条件下果胶的得率达24.81%。

关键词: 黄秋葵, 果胶, 微波辅助提取, 响应面分析

Abstract:

Response surface methodology was applied to optimize the extraction process of pectin from okra by microwaveassisted
hydrochloric acid extraction and ethanol precipitation method. A mathematical model describing the extraction
efficiency of pectin as a function of microwave power, microwave radiation time, extraction temperature and solvent pH was
established by Box-Behnken design. The optimal process parameters for microwave-assisted extraction of pectin from okra
by acid extraction and ethanol precipitation under the conditions of 60 s microwave treatment and 1:20 of solid-to-liquid
ratio (g/mL) were 590 W microwave power, solvent pH 2.9, 30 min and 74 ℃. Under these conditions, the pectin yield
reached up to 24.81%.

Key words: okra, pectin, microwave-assisted extraction, response surface methodology