正交试验优化酵母多糖锌配合物的制备及其对尿素的吸附性能

doi:10.7506/spkx1002-6630-201314012

食品科学

正交试验优化酵母多糖锌配合物的制备及其对尿素的吸附性能

李兴艳，张丙云，尚永彪

1.西南大学食品科学学院，重庆 400716；2.兰州理工大学生命科学与工程学院，甘肃兰州 730050；
3.农业部农产品贮藏保鲜质量安全评估实验室(重庆)，重庆 400716；4. 重庆市特色食品工程技术研究中心，重庆 400716

出版日期:2013-07-25 发布日期:2013-08-02

Preparation of Yeast Polysaccharide-Zn Complex and Its Adsorption Performance for Urea

LI Xing-yan，ZHANG Bing-yun，SHANG Yong-biao

1. College of Food Science, Southwest University, Chongqing 400716, China；
2. School of Life Science and Engineering, Lanzhou University of Science and Technology, Lanzhou 730050, China；
3. Quality and Safety Risk Assessment Laboratory of Products Preservation (Chongqing), Ministry of Agriculture, Chongqing 400716, China；
4. Chongqing Special Food Programme and Technology Research Center, Chongqing 400716, China

Online:2013-07-25 Published:2013-08-02

摘要/Abstract

摘要：

为酵母多糖应用拓宽范围，以酵母多糖和硫酸锌为原料制备酵母多糖锌配合物，探讨了反应时间、锌的添加量、反应温度、pH值4个因素对配合物制备的影响。通过正交试验，确定制备锌配合物的最优条件。以尿素为吸附对象，研究酵母多糖锌配合物的吸附性能，考察了时间、温度、pH值、尿素质量浓度4个因素对配合物吸附尿素的影响，通过正交试验，确定酵母多糖锌配合物吸附尿素的最优条件。结果表明：酵母多糖锌配合物制备的最佳条件为温度55℃、反应时间2h、0.1mol/L硫酸锌的添加量10%、pH6.5，在该条件下所得到的酵母多糖和锌离子结合率为98.97%。酵母多糖锌配合物对尿素的最佳吸附条件为：温度30℃、pH6.0、时间135min、尿素的初始质量浓度2.25mg/mL，在该条件下对尿素的吸附率为97.27%。此时酵母多糖和锌离子的结合率较高，且该配合物对尿素具有很好的吸附作用。

关键词: 酵母多糖, 锌离子, 酵母多糖锌配合物, 制备, 尿素, 吸附

Abstract:

Yeast cell wall polysaccharide contains some groups that can combine metal ions for executing bioactive
functions such as immunity. Yeast polysaccharide-Zn complex was prepared in this study to explore the absorption
performance of urea and to extend the application of yeast polysaccharide. Yeast polysaccharide-Zn complex is composed
of yeast polysaccharide and zinc sulfate. The influential factors of complex preparation such as reaction time, zinc addition
amount, reaction temperature and reaction pH were investigated. The optimal conditions of zinc complex preparation were
explored by orthogonal tests using urea adsorption as the evaluation index. The results indicated that the optimal preparation
conditions of yeast polysaccharide-Zn complex required reaction temperature of 55 ℃, reaction time of 2 h, addition of 10%
of 0.1 mol/L zinc sulfate, and reaction pH of 6.5. Under the optimal reaction conditions, the binding rate was up to 98.97%.
The optimal adsorption conditions of yeast polysaccharide-Zn complex for urea were reaction temperature of 30 ℃, reaction
pH of 6.0, reaction time of 135 min and initial urea concentration of 2.25 mg/mL. Under these optimal conditions, the
adsorption rate was up to 97.27%. The higher binding rate between yeast polysaccharide and zinc ion can provide the better
adsorption for urea.

Key words: yeast polysaccharide, zinc ion, yeast polysaccharide-Zn, preparation, urea, adsorption

李兴艳，张丙云，尚永彪. 正交试验优化酵母多糖锌配合物的制备及其对尿素的吸附性能[J]. 食品科学, doi: 10.7506/spkx1002-6630-201314012.

LI Xing-yan，ZHANG Bing-yun，SHANG Yong-biao. Preparation of Yeast Polysaccharide-Zn Complex and Its Adsorption Performance for Urea[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201314012.

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正交试验优化酵母多糖锌配合物的制备及其对尿素的吸附性能

Preparation of Yeast Polysaccharide-Zn Complex and Its Adsorption Performance for Urea

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