响应面分析法优化雪灵芝黄酮提取工艺

doi:10.7506/spkx1002-6630-201314018

食品科学

响应面分析法优化雪灵芝黄酮提取工艺

王昌涛，孙啸涛，周雪

北京工商大学北京市植物资源研究开发重点实验室，北京 100048

出版日期:2013-07-25 发布日期:2013-08-02

Optimization of Extraction Process for Flavonoids from Arenaria kansuensis Maxim. by Response Surface Methodology

WANG Chang-tao，SUN Xiao-tao，ZHOU Xue

Beijing Key Laboratory of Plant Resources Research and Development, Beijing Technology and Business University,Beijing 100048, China

Online:2013-07-25 Published:2013-08-02

摘要/Abstract

摘要：

利用响应面分析法对雪灵芝中黄酮类物质的提取工艺进行优化。通过单因素试验分别考察乙醇体积分数、提取时间、提取温度和料液比对雪灵芝黄酮提取率的影响，选取适当的试验因素水平，利用Design Expert软件和Box-Behnken设计法设计响应面试验，对各个因素的显著性和交互作用进行分析。结果表明：雪灵芝黄酮醇提工艺的最佳条件为料液比1:40(g/mL)、乙醇体积分数68.6%、提取温度66℃、提取时间0.83h(50min)，在该条件下验证总黄酮提取率达到5.07mg/g，接近于预测值5.10mg/g。研究表明，优化得到的回归模型具有良好的预测能力。

关键词: 雪灵芝, 提取, 响应面分析法, 总黄酮

Abstract:

The optimal extraction conditions for flavonoids from Arenaria kansuensis Maxim. were determined using
response surface methodology (RSM). On the basis of single-factor tests, the effects of ethanol concentration, extraction
duration, extraction temperature, and liquid-material ratio on extraction rate were explored. A regression model describing
extraction rate of flavonoids as a function of three variables was established with the Design-Expert software according to
the Box-Behnken design principle and experimental data. Quadratic regression analysis was used to establish a mathematical
model describing flavonoids extraction. The optimal extraction conditions for flavonoids from Arenaria kansuensis Maxim.
were material-liquid ratio of 1:40 (g/mL), ethanol concentration of 68.6%, extraction temperature of 66 ℃ and extraction
time of 0.83 h. Under the optimal extraction conditions, the maximum extraction yield of flavonoids was 5.07 mg/g, which
was close to the predicted value of 5.10 mg/g. Therefore, the established regression model has good prediction capability.

Key words: Arenaria kansuensis Maxim., extraction, response surface methodology (RSM), flavonoids

王昌涛，孙啸涛，周雪. 响应面分析法优化雪灵芝黄酮提取工艺[J]. 食品科学, doi: 10.7506/spkx1002-6630-201314018.

WANG Chang-tao，SUN Xiao-tao，ZHOU Xue. Optimization of Extraction Process for Flavonoids from Arenaria kansuensis Maxim. by Response Surface Methodology[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201314018.

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响应面分析法优化雪灵芝黄酮提取工艺

Optimization of Extraction Process for Flavonoids from Arenaria kansuensis Maxim. by Response Surface Methodology

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