食品科学

• 工艺技术 • 上一篇    下一篇

响应面优化超声波辅助提取大麦β-葡聚糖工艺

刘振春,刘春萌,苏 彤   

  1. 1.吉林农业大学食品与科学工程学院,吉林 长春 130118;2.长春职业技术学院,吉林 长春 130033
  • 出版日期:2013-07-25 发布日期:2013-08-02

Process Optimization for Ultrasonic-Assisted Extraction of Barley β-glucan by Response Surface Analysis

LIU Zhen-chun,LIU Chun-meng,SU Tong   

  1. 1. College of Food Science and Technology, Jilin Agricultural University, Changchun 130118, China;
    2. Changchun Vocational Institute of Technology, Changchun 130033, China
  • Online:2013-07-25 Published:2013-08-02

摘要:

以大麦为原料,用响应面法对超声波辅助提取大麦β-葡聚糖的工艺进行研究。在单因素试验的基础上,以超声时间、提取温度、pH值为自变量,β-葡聚糖得率为响应值,紫外-可见分光光度法检测β-葡聚糖含量,通过响应面分析法对提取工艺进行优化。试验得到的最佳提取工艺条件为pH10.35、提取温度83.34℃、超声时间12.8min,在此条件下进行提取,得率为6.81%,理论值为6.99%。由响应面分析法优化的β-葡聚糖提取最佳工艺所产生的实际得率与理论得率接近,可为实际生产提供理论依据。

关键词: 大麦&beta, -葡聚糖, 超声波, 响应面

Abstract:

Barleys were used as the raw material to explore the ultrasonic-assisted extraction of β-glucan. Response surface
methodology (RSM) was used to optimize the effects of extraction temperature, ultrasonic time and pH as well as their crossinteractions
on extraction rate of β-glucan. The quantification of β-glucan was conducted using a UV spectrophotometer. A
regression equation was established to obtain the optimal extraction parameters: ultrasonic time of 12.8 min, extraction pH
of 10.35, and extraction temperature of 83.34 ℃. Under this optimal extraction conditions, the extraction rate of β-glucan
was 6.81%, which revealed a close agreement with the predicted value. Ultrasonic-assisted extraction can be applied for the
production of β-glucan.

Key words: barley &beta, -glucan;ultrasound;response surface methodology