食品科学

• 工艺技术 • 上一篇    下一篇

快速溶剂萃取仪提取葡萄籽中原花青素条件优化

袁 琳,袁春龙,胡立志,张世杰,杨晓雁,杨 健   

  1. 西北农林科技大学葡萄酒学院,陕西 杨凌 712100
  • 出版日期:2013-07-25 发布日期:2013-08-02

Optimization of Extraction Conditions for Proanthocyanidins from Grape Seeds by Accelerated Solvent Extraction

YUAN Lin,YUAN Chun-long,HU Li-zhi,ZHANG Shi-jie,YANG Xiao-yan,YANG Jian   

  1. College of Enology, Northwest A & F University, Yangling 712100, China
  • Online:2013-07-25 Published:2013-08-02

摘要:

利用快速溶剂萃取仪优化提取新品种‘媚丽’葡萄籽中原花青素。结果表明:利用响应面优化方法,得到最佳萃取条件为提取时间13min、提取压强1161psi、甲醇体积分数80%、提取温度80℃,此时原花青素提取率的理论预测值为6.817%,重复提取3次,测得提取率为6.286%,相对误差为7.79%,纯度41.02%。

关键词: 葡萄籽原花青素, 快速溶剂萃取, Box-Behnken试验设计

Abstract:

Response surface methodology was applied to optimize the extraction of proanthocyanidins from seeds of
the new grape cultivar “Meili” using an accelerated solvent extraction (ASE) system. The optimal extraction conditions
were found to be 13 min, 1161 psi, 80% and 80 ℃ for extraction time, pressure, methanol concentration and temperature,
respectively. The maximum predicted extraction yield of proanthocyanidins under the optimized conditions was 6.817%
indicating a relative error of 7.79% as compared with the actual value of 6.286% obtained after the third repeated extraction.
The purity of proanthocyanidins under the optimized conditions was 41.02%.

Key words: proanthocyanidins (PCs), ASE, Box-Behnken experimental design