食品科学

• 工艺技术 • 上一篇    下一篇

响应曲面法优化大高良姜黄酮酶法提取工艺

彭 晶,杨 颖,牛付阁,李清宇,贾琳斐,段玉峰,郭 行   

  1. 陕西师范大学食品工程与营养科学学院,陕西 西安 710062
  • 出版日期:2013-07-25 发布日期:2013-08-02

Optimization of Enzymatic Extraction of Flavonoids from Galangal Rhizomes (Alpinia galanga)

PENG Jing,YANG Ying,NIU Fu-ge,LI Qing-yu,JIA Lin-fei,DUAN Yu-feng,GUO Xing   

  1. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China
  • Online:2013-07-25 Published:2013-08-02

摘要:

为研究大高良姜活性成分,选用纤维素酶法提取大高良姜黄酮,并选取酶添加量、酶解时间、酶解温度和酶解pH值4个因素进行单因素试验,在此基础上以4个因素为自变量,提取率为响应值,采用Box-Behnken试验设计和响应面分析法,建立数学模型确定大高良姜黄酮提取的最佳工艺条件为酶添加量30.0U/mL、酶解温度56.6℃、pH5.14、酶解时间1.71h,在此条件下,大高良姜总黄酮的提取率为5.08%。

关键词: 大高良姜, 黄酮, 纤维素酶法提取, 响应曲面法

Abstract:

The cellulose-assisted extraction of flavonoids from galangal rhizomes (Alpinia galanga) was optimized by
response surface methodology based on Box-Behnken design. The extraction efficiency of flavonoids was investigated with
respect to four variables including enzyme dosage, hydrolysis time, temperature and pH, and a mathematical model was
developed. The optimal enzyme dosage, temperature, initial pH and time for extracting flavonoids from galangal rhizomes
were 30.0 U/mL, 56.6 ℃, 5.14 and 1.71 h, respectively. The extraction yield of flavonoids under these conditions was 5.08%.

Key words: galangal rhizomes, flavonoids, cellulose-assisted extraction, response surface methodology