食品科学

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畜禽产品中脱氧雪腐镰刀菌烯醇和T-2毒素残留分析

邹忠义,贺稚非,李洪军,韩鹏飞,孟 晓,周 芳,罗理勇   

  1. 1.西南大学食品科学学院,重庆 400715;2.公安海警学院后勤管理系,浙江 宁波 315801
    3.西南大学 重庆市特色食品工程技术研究中心,重庆 400715;
  • 出版日期:2013-07-25 发布日期:2013-08-02

Analysis of Residual Deoxynivalenol and T-2 Toxin in Livestock and Poultry Products

ZOU Zhong-yi,HE Zhi-fei,LI Hong-jun,HAN Peng-fei,MENG Xiao,ZHOU Fang,LUO Li-yong   

  1. 1. College of Food Science, Southwest University, Chongqing 400715, China;
    2. Department of Logistics Management, China Maritime Police Academy, Ningbo 315801, China;
    3. Chongqing Special Food Programme and Technology Research Center, Southwest University, Chongqing 400715, China
  • Online:2013-07-25 Published:2013-08-02

摘要:

目的:分析市售畜禽产品中脱氧雪腐镰刀菌烯醇(DON)和T-2毒素的残留情况。方法:试样经乙腈提取、正己烷脱脂、HLB柱净化,用高效液相色谱-质谱(HPLC-MS/MS)测定,外标法定量。结果:所有受检猪背脊肌肉、猪肝、猪肾、鸡胸肉、鸡腿肉、鸡翅肉和肉制品样品中均未发现DON残留,猪背脊脂肪样品中DON残留阳性率为28.75%,最高残留量为0.4265μg/kg;所有受检猪肝、猪肾和肉制品样品中均未发现T-2毒素残留,猪背脊肌肉、猪背脊脂肪、鸡胸肉、鸡腿肉、鸡翅肉样品中T-2毒素残留阳性率范围为8.33%~57.14%,最高残留量范围为0.0704~0.4515μg/kg。结论:在部分受检畜禽样品中检测到了DON和T-2毒素痕量残留。

关键词: 畜禽产品, 脱氧雪腐镰刀菌烯醇, T-2毒素, 残留, 高效液相色谱-质谱, 分析

Abstract:

Objective: To analyze residual deoxynivalenol (DON) and T-2 toxin in products of livestock and poultry.
Methods: Residual DON and T-2 toxin in samples were extracted with acetonitrile, defatted with n-hexane, cleaned up on an
HLB cartridge, and analyzed by high performance liquid chromatography coupled with tandem mass spectrometry (HPLCMS/
MS) and quantified by an external standard method. Results: Residual DON was not observed in all tested samples
of pig dorsal muscle, pig liver, pig kidney, chicken breast, chicken thighs, chicken wings and meat products, but detected
in 28.75% of pig back fat, and the maximum residue was up to 0.4265 μg/kg. No residual T-2 toxin was detected in all
tested samples of pig liver, pig kidney and meat products, but detected in 8.33%–57.14% of pig dorsal muscle, pig back fat,
chicken breast, chicken thighs and chicken wings, and the maximum contents were 0.0704–0.4515 μg/kg. Conclusion: Trace
residues of DON and T-2 toxin can be detected in partial tested samples of livestock and poultry products.

Key words: products of livestock and poultry;deoxynivalenol;T-2 toxin, residue;high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS);analysis