食品科学

• 工艺技术 • 上一篇    下一篇

香蕉皮黄酮的分离纯化及抑菌活性研究

顾采琴,谢琳琳,张织芬,朱冬雪,刘 鹏,赖雅平,曾庆祝   

  1. 广州大学化学化工学院食品系,广东 广州 510006
  • 出版日期:2013-08-25 发布日期:2013-09-03

Purification and Antimicrobial Activity of Total Flavonoid from Banana Peel

GU Cai-qin,XIE Lin-lin,ZHANG Zhi-fen,ZHU Dong-xue,LIU Peng,LAI Ya-ping,ZENG Qing-zhu   

  1. Department of Food, School of Chemistry and Chemical Engineering, Guangzhou University, Guangzhou 510006, China
  • Online:2013-08-25 Published:2013-09-03

摘要:

以香蕉皮为原料,通过比较X-5、AB-8、H103、D101大孔树脂对香蕉皮黄酮静态吸附率和解吸率的影响,筛选出适宜分离纯化香蕉皮黄酮的大孔树脂,并通过单因素和多因素正交试验优化分离纯化工艺参数,同时测定了香蕉皮黄酮纯化前后对3种常见微生物的抑菌活性。结果表明:H103树脂为分离纯化香蕉皮黄酮的最佳树脂,其对香蕉皮黄酮的最大吸附量为3.66mg/g,平衡吸附时间约3h,解吸率达89.11%,解吸平衡时间约2.5h;H103分离纯化香蕉皮黄酮的动态吸附和解吸最佳工艺参数为上样液pH7.0、上样流速2.0mL/min、洗脱流速3.0mL/min、洗脱剂乙醇体积分数75%,此条件下黄酮回收率为72.50%,所得黄酮纯度为73%。香蕉皮黄酮对酵母、枯草芽孢杆菌和大肠杆菌均有一定的抑制作用,黄酮纯化后的抑菌效果优于纯化前。

关键词: 香蕉皮黄酮, 大孔树脂, 分离纯化, 抑菌活性

Abstract:

The best macroporous resin for purification of banana peel flavonoid (BPF) was selected from X-5, AB-8, H103 and
D-101 by static adsorption and desorption capacities. H103 was identified as the best resin for BPF purification. The adsorption
equilibrium was reached in about 3 h and the maximum adsorption quantity for BPF was 3.66 mg/g, while the time required to
reach desorption equilibrium was about 2.5 h, yielding a desorption percentage of 89.11%. The optimal conditions for purifying
BPF by dynamic adsorption and desorption on H103 were determined as 7.0, 2.0 mL/min, 3.0 mL/min and 75% ethanol for pH
sample loading flow rate, desorption flow rate and desorbent, respectively. Under these conditions, the recovery and purity of
BPF were 72.50% and 73%, respectively. BPF could inhibit the growth of Candida albicans, Escherichia coli, Bacillus subtilis,
and purified BPF had better antimicrobial activity.

Key words: banana peel flavonoid, macroporous resin, purification, antimicrobial activity