食品科学

• 基础研究 • 上一篇    下一篇

酸性电解水对纯培养及食品中食源性致病菌杀菌效果比较研究

林 婷,王敬敬,潘迎捷,赵 勇*   

  1. 上海海洋大学食品学院,上海水产品加工及贮藏工程技术研究中心,上海 201306
  • 出版日期:2013-08-15 发布日期:2013-09-03
  • 通讯作者: 赵 勇
  • 基金资助:

    上海市科学技术委员会部分地方院校能力建设项目(11310501100);上海市科委工程中心建设项目(11DZ2280300);
    上海市教委重点学科建设项目(J50704)

Comparison of the Bactericidal Activity of Acidic Electrolyzed Water against Foodborne Pathogenic Bacteria in Pure Culture and Foods

LIN Ting,WANG Jing-jing,PAN Ying-jie,ZHAO Yong*   

  1. Shanghai Engineering Research Center of Aquatic-product Processing and Preservation, College of Food Science and Technology,
    Shanghai Ocean University, Shanghai 201306, China
  • Online:2013-08-15 Published:2013-09-03
  • Contact: 赵 勇

摘要:

比较研究不同有效氯质量浓度的酸性电解水对纯培养单增李斯特菌和副溶血性弧菌,以及人工接种到黄瓜上的单增李斯特菌和副溶血性弧菌处理不同时间后的杀灭效果。结果表明:酸性电解水分别对在纯培养条件下及在黄瓜上的2种食源性致病菌均具有很强的杀灭效果,其杀菌效果随着有效氯(ACC)质量浓度和处理时间的增加而增强。对纯培养物的杀菌实验表明:当酸性电解水ACC质量浓度为30mg/L时,0.5min内可以完全杀灭副溶血性弧菌;当ACC质量浓度为40mg/L时,0.5min内可以完全杀灭单增李斯特菌。酸性电解水处理上述黄瓜时,当ACC质量浓度为40mg/L时,1.5min内可将黄瓜表面的副溶血性弧菌全部杀灭;当ACC质量浓度为50mg/L时,2.5min内可杀灭黄瓜表面的单增李斯特菌约5(lg(CFU/g))。检测使用后的酸性电解水废液,发现其中无任何微生物残留,排放后不会对环境造成二次污染。

关键词: 酸性电解水, 单增李斯特菌, 副溶血性弧菌, 黄瓜, 杀菌效果

Abstract:

This study examined the efficacy of acidic electrolyzed water (AEW) with different concentrations of available
chlorine concentration (ACC) for inactivating Listeria monocytogenes and Vibrio parahaemolyticus in pure culture and on
surface-inoculated cucumber. AEW had strong bactericidal activity against pure-cultured and surface-inoculated strains
of Listeria monocytogenes and Vibrio parahaemolyticus which became stronger with increasing AEW concentration and
treatment time. Complete inactivation of pure Vibrio parahaemolyticus was achieved when the pathogen was treated with
AEW at an ACC concentration of 30 mg/L for 0.5 min, while the conditions for complete inactivation of pure Listeria
monocytogenes were 40 mg/L ACC in AEW and 0.5 min. The ACC concentration and time for complete inactivation of
surface-inoculated Vibrio parahaemolyticus were 40 mg/L and 1.5 min, respectively. After 2.5 min of treatment at an ACC
concentration of 50 mg/L, the population of surface-inoculated Listeria monocytogenes was reduced by approximately 5
(lg(CFU/g)). The used AEW may not cause secondary environmental pollution, since no residual pathogens were detected.

Key words: acidic electrolyzed water, Listeria monocytogenes, Vibrio parahaemolyticus, cucumber, bactericidal activity

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