食品科学 ›› 2013, Vol. 34 ›› Issue (21): 315-320.doi: 10.7506/spkx1002-6630-201321063

• 营养卫生 • 上一篇    下一篇

膳食中晚期糖基化终产物对大鼠肾脏的影响

张双凤,苏亚儒,李巨秀*   

  1. 西北农林科技大学食品科学与工程学院,陕西 杨凌 712100
  • 收稿日期:2013-06-29 修回日期:2013-10-10 出版日期:2013-11-15 发布日期:2013-10-28
  • 通讯作者: 李巨秀 E-mail:656612204@qq.com
  • 基金资助:

    国家自然科学基金项目(31101217);西北农林科技大学科技创新专项(QN 2012034)

Effect of Dietary Advanced Glycation End Products on Diabetic Rat Kidney

ZHANG Shuang-feng,SU Ya-ru,LI Ju-xiu*   

  1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
  • Received:2013-06-29 Revised:2013-10-10 Online:2013-11-15 Published:2013-10-28
  • Contact: LI Ju-xiu E-mail:656612204@qq.com

摘要:

以SD大鼠为实验动物,建立糖尿病大鼠模型,用含量不同的晚期糖基化终产物(AGEs)饲料饲喂糖尿病鼠和正常鼠8周,研究大鼠肾脏系数、血肌酐、血清荧光值、肾组织超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)的变化,观察AGEs摄入对大鼠肾脏的影响,并对其机制进行探讨,以评估膳食中AGEs的安全性。结果表明:AIN-93G饲料经过高温烘烤(125℃,3h),荧光性AGEs增加4倍左右。从第4周到第8周,AGEs实验组大鼠的肾脏损伤加剧,大鼠肾脏氧化应激加剧。喂养至第8周,与低AGEs饲料糖尿病鼠组相比,高AGEs饲料糖尿病鼠组的肾脏系数((14.84±2.09)g/kg)、血肌酐浓度((40.55±3.51)μmol/L)、血清荧光值((898.60±58.07)AU)、MDA含量((5.05±0.42)nmol/mg pro)均显著升高(P<0.05)、SOD活力((89.11±11.07)U/mg pro)、GSH含量((4.12±0.58)mg/g pro)均降低,但差异不显著(P>0.05);与低AGEs饲料正常鼠组相比,高AGEs饲料正常鼠组的肾脏系数、血肌酐浓度、血清荧光值、MDA含量分别上升了11.86%、31.76%、44.49%、163.02%,SOD活力、GSH含量分别下降了50.56%、65.23%。因此,高AGEs饲料促进糖尿病鼠和正常鼠的肾功能恶化,作用机制可能与氧化应激有关。限制AGEs的摄入,可以作为延缓糖尿病肾病恶化的措施之一。

关键词: 晚期糖基化终产物, 糖尿病, 肾病, 氧化应激

Abstract:

Forty-eight SD rats were used as experimental animals to establish diabetic rat models by intraperitoneal injection
of streptozotocin. Changes in kidney coefficient, serum creatinine, serum fluorescence, superoxide dismutase (SOD)
in kidney tissue, malondialdehyde (MDA), and reduced glutathione (GSH) were evaluated on rats after feeding with advanced
glycation products at various concentrations for 8 weeks. In addition, the effect and mechanism of dietary AGEs
on rat kidney were investigated to evaluate the safety of AGEs in diet. The results showed that fluorescent AGEs of AIN-
93G diet revealed 4 fold increase after baking at high temperature (125 ℃, 3 h). Kidney injury caused by AGEs was enhanced
during weeks 4 to 8. Approximately 8 weeks later, compared with low AGEs diet, kidney coefficient ((14.84 ± 2.09) g/kg),
serum creatinine concentration ((40.55 ± 3.51) μmol/L), serum fluorescence value ((898.6 ± 58.07) AU) and MDA
content ((5.05 ±0.42) nmol/mg pro) in diabetic rats fed on high AGE diet were significantly higher (P < 0.05), while SOD
activity ((89.11 ± 11.07) U/mg pro) and GSH level ((4.12 ± 0.58) mg/g pro) were decreased without significant difference
(P >0.05); compared with low AGEs, kidney coefficient, serum creatinine concentration, serum fluorescence value and
MDA content of normal rats fed on high AGEs diet were increased by 11.86%, 31.76%, 44.49% and 163.02%, respectively,
while SOD activity and GSH content were decrease by 50.56% and 65.23%, respectively. In conclusion, high AGEs diet can
promote the deterioration of renal function in rats and its mechanism may be associated with oxidative stress. Limited intake
of AGEs can be introduced as one of the strategies to slow down the deterioration of diabetic nephropathy.

Key words: advanced glycation end products, diabetes, nephropathy, oxidative stress

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