青钱柳多糖在体外消化模型中的消化与吸收

doi:10.7506/spkx1002-6630-201321006

食品科学 ›› 2013, Vol. 34 ›› Issue (21): 24-29.doi: 10.7506/spkx1002-6630-201321006

青钱柳多糖在体外消化模型中的消化与吸收

闵芳芳，聂少平，万宇俊，谢明勇*

南昌大学食品科学与技术国家重点实验室，江西南昌 330047

收稿日期:2013-06-14 修回日期:2013-10-08 出版日期:2013-11-15 发布日期:2013-10-28
通讯作者: 谢明勇 E-mail:myxie@ncu.edu.cn
基金资助:
国家自然科学基金项目(31130041)；“十二五”国家科技支撑计划项目(2012BAD33B06)

in vitro Digestion and Absorption of Polysaccharide from Cyclocarya paliurus Leaves

MIN Fang-fang，NIE Shao-ping，WAN Yu-jun，XIE Ming-yong*

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Received:2013-06-14 Revised:2013-10-08 Online:2013-11-15 Published:2013-10-28
Contact: XIE Ming-yong E-mail:myxie@ncu.edu.cn

摘要/Abstract

摘要：

采用水提醇沉法得到青钱柳多糖并测定其基本理化性质，同时，采用体外模拟人体胃肠道的消化模型，通过测定体外消化后产物的分子质量、还原糖含量和游离单糖含量，研究青钱柳多糖在体外模拟胃和胃肠道中的消化与吸收的情况。结果表明：青钱柳多糖的中性糖含量为31.17%，糖醛酸含量为38.44%，是一种果胶类的多糖；青钱柳多糖在体外模拟胃中会产生絮凝沉淀，而在体外模拟胃肠道的肠道消化阶段絮凝沉淀消失；青钱柳多糖经模拟胃和胃肠道消化后上清液和沉淀物分子质量没有发生变化，与多糖水溶液相比，多糖胃电解质溶液的高效凝胶渗透色谱的保留时间提前，同时，消化产物的还原糖和游离单糖含量也没有改变。结论：青钱柳多糖在体外模拟胃和模拟胃肠道均未被消化；但体外模拟胃中酸性环境和胃电解质溶液体系分别对青钱柳多糖的稳定性和构象产生了一定的影响。

关键词: 青钱柳多糖, 模拟胃肠道模型, 体外消化, 体外吸收

Abstract:

Polysaccharides were isolated from Cyclocarya paliurus leaves by water extraction and ethanol precipitation and
their physicochemical properties were determined. The digestion and absorption of the polysaccharides were studied in vitro by
employing a simulated upper gastrointestinal model and analyzing molecular weight, reducing sugar and free monosaccharide
contents of digested products at different digestion times. The results indicated that the polysaccharide extract contained 31.17%
neutral polysaccharides and 38.44% uronic acid and was considered as pectic polysaccharides. The polysaccharides from
Cyclocarya paliurus leaves formed flocculation in in vitro gastric digestion model, while the flocculation was eliminated in in vitro
gastrointestinal model. The molecular weights of digestion products at different digestion times remained unchanged in in vitro
simulated gastric and upper gastrointestinal model. Compared with aqueous solution, the retention time of these polysaccharides
dissolved in the gastric juice was shorter in high performance gel permeation chromatography. Meanwhile, reducing sugar and
free monosaccharide contents of digested products also kept unchanged in simulated gastric and upper gastrointestinal models.
Therefore, the polysaccharides from Cyclocarya paliurus leaves were not digested in vitro, but to some extent, their stability and
configuration were affected by acidic environment in in vitro simulated gastric model and gastric electrolytes, respectively.

Key words: polysaccharides from Cyclocarya paliurus leaves, in vitro simulated upper gastrointestinal model, in vitro digestion, in vitro absorption

中图分类号:

TS201.2

闵芳芳，聂少平，万宇俊，谢明勇*. 青钱柳多糖在体外消化模型中的消化与吸收[J]. 食品科学, 2013, 34(21): 24-29.

MIN Fang-fang，NIE Shao-ping，WAN Yu-jun，XIE Ming-yong*. in vitro Digestion and Absorption of Polysaccharide from Cyclocarya paliurus Leaves[J]. FOOD SCIENCE, 2013, 34(21): 24-29.

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青钱柳多糖在体外消化模型中的消化与吸收

in vitro Digestion and Absorption of Polysaccharide from Cyclocarya paliurus Leaves

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