食品科学 ›› 2013, Vol. 34 ›› Issue (22): 64-69.doi: 10.7506/spkx1002-6630-201322013

• 工艺技术 • 上一篇    下一篇

超临界CO2萃取枣核多酚工艺优化及其生物活性

刘杰超,张春岭,刘 慧,郑晓伟,王思新,焦中高   

  1. 中国农业科学院郑州果树研究所,河南 郑州 450009
  • 收稿日期:2013-06-14 修回日期:2013-10-25 出版日期:2013-11-25 发布日期:2013-12-05
  • 通讯作者: 焦中高 E-mail:jchzz@sina.com
  • 基金资助:

    河南省科技攻关计划重点项目

Supercritical CO2 Extraction and Bioactivity of Polyphenols from Chinese Jujube Seeds

LIU Jie-chao,ZHANG Chun-ling,LIU Hui,ZHENG Xiao-wei,WANG Si-xin,JIAO Zhong-gao   

  1. Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China
  • Received:2013-06-14 Revised:2013-10-25 Online:2013-11-25 Published:2013-12-05
  • Contact: JIAO Zhong-Gao E-mail:jchzz@sina.com

摘要:

采用超临界CO2萃取枣核中的多酚物质,并对得到的枣核提取物的DPPH自由基清除能力和对α-淀粉酶、α-葡萄糖苷酶及透明质酸酶的抑制活性等进行分析测定。结果表明:以提取率为考察指标,超临界CO2萃取枣核多酚类物质的最佳提取工艺条件为萃取时间2.5h、萃取压力35MPa、萃取温度50℃、萃取次数2次;枣核多酚提取物对DPPH自由基具有较强的清除作用,并可有效抑制α-淀粉酶、α-葡萄糖苷酶及透明质酸酶的活性,在实验质量浓度范围内其最大清除率和抑制率分别可达到93.52%、86.67%、98.73%和96.86%,IC50值分别为6.16、9.81、7.29mg/mL和5.59mg/mL(折合成枣核质量浓度)。相关性分析表明,枣核提取物的自由基清除活性和对α-淀粉酶、α-葡萄糖苷酶及透明质酸酶的抑制活性均与其中的总多酚含量呈极显著正相关关系,但不同萃取条件得到的枣核提取物的生物活性并不一定与其中的总酚含量一一对应,获得高提取率所需工艺条件与获得高活性提取物所需工艺条件可能不同。

关键词: 枣核;多酚;超临界CO2提取;DPPH自由基;&alpha, -淀粉酶;&alpha, -葡萄糖苷酶;透明质酸酶

Abstract:

Polyphenols from Chinese jujube seeds were extracted by supercritical carbon dioxide extraction and their
DPPH radical scavenging activity and inhibitory activities against α-amylase, α-glucosidase and hyaluronidase were
evaluated. The optimal extraction conditions that provided maximum extraction efficiency were determined as 2.5 h,
35 MPa, 50 ℃ and 2 for time, pressure, temperature and extraction number, respectively. The extracted polyphenols
possessed strong DPPH radical scavenging activity and could effectively inhibit α-amylase, α-glucosidase and hyaluronidase
and the corresponding inhibition rates were 93.52%, 86.67%, 98.73% and 96.86% over the concentration
range tested with IC50 values of 6.16, 9.81, 7.29 mg/mL and 5.59 mg/mL (expressed as the weight of jujube seeds),
respectively. Correlation analysis suggested an extremely significant positive correlation between the content of
polyphenols and the activities to scavenge DPPH free radical and inhibit three enzymes in jujube seed extracts.
Interestingly, it was also found that the optimal conditions for high yield of polyphenols were not in accordance
with those obtained for high activity, suggesting that the extraction conditions pressure, temperature, duration
and number of repeats could not only affect the yield of polyphenols, but the biological activity as well.

Key words: jujube seed;polyphenol;supercritical CO2 extraction;DPPH radical;&alpha, -amylase;&alpha, -glucosidase;hyaluronidase

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