关键词: 大粒车前子, 多糖, 乙酰化, 树突状细胞

Abstract:

Acetylated polysaccharide from the seeds of Plantago asiatica L. (Ac-PLP) which provides materials for studying the
structure-activity relationship of plant polysaccharides was prepared by using acetic anhydride as acetylating agent. Factors
influencing the modification reaction, such as acetic anhydride dosage, temperature and reaction time, were investigated.
The optimal reaction conditions were determined using orthogonal array design as 10% of acetic anhydride, 30 ℃ and
2.5 h. Further in vitro investigation demonstrated that Ac-PLPs with a substitution degree from 0.06 to 0.1 promoted
IL-12p70 secretion by dendritic cells and the strongest effect was observed when the degree of substitution was about 0.08.
The results in the current study reveal that acetylation can be an alternative way to modify PLPs and provide a theoretic basis
for the development of functional products with PLPs.

Key words: seeds of Plantago asiatica L., polysaccharide, acetylation, dendritic cells