食品科学

• 工艺技术 • 上一篇    下一篇

二次回归中心组合法优化外源纤维素酶酶解提取香草兰青豆荚香兰素工艺

莫丽梅,张彦军,谷风林,徐 飞,陆敏泉   

  1. 1.海南大学食品学院,海南 海口 570228;2.中国热带农业科学院香料饮料研究所,海南 万宁 571533;
    3.农业部香辛饮料作物遗传资源利用重点实验室,海南 万宁 571533
  • 出版日期:2013-09-25 发布日期:2013-09-27

Quadratic Regression Central Composite Method for the Optimization of Enzymatic Extraction of Vanillin from Vanilla (Vanilla planifolia Andrews) Pods with Exogenous

MO Li-mei,ZHANG Yan-jun,GU Feng-lin,XU Fei,LU Min-quan   

  1. 1. College of Food Science, Hainan University, Haikou 570228, China;
    2. Institute of Spice and Beverage Research, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, China;
    3. Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture, Wanning 571533, China
  • Online:2013-09-25 Published:2013-09-27

摘要:

为优化纤维素酶酶解天然香草兰青豆荚工艺,本实验采用二次回归中心组合方法考察外源添加纤维素酶处理对香草兰青豆荚香兰素含量的影响。结果表明:最佳酶解工艺条件为香草兰青豆荚10g、酶解温度53℃、纤维素酶添加量26.9mg、酶解时间16h,此条件下天然香草兰青豆荚中香兰素含量为2.18%,相比于传统发酵乙醇回流提取法的1.97%,香兰素含量提高0.21%。

关键词: 香兰素, 纤维素酶, 条件优化

Abstract:

This study was conceived to develop an optimized procedure for enzymatic extraction of vanillin from vanilla green pods
with exogenous cellulase. For this, quadratic regression central composite design combined with response surface methodology was
employed to investigate the effects of enzymatic hydrolysis temperature, cellulase dosage and enzymatic hydrolysis time on vanillin
content. The results showed that the optimum conditions of vanilla green pods, enzymatic hydrolysis temperature, cellulase and
enzymatic hydrolysis time were 10 g, 53 ℃, 26.9 mg and 16 h, respectively. The maximum vanillin content under these conditions
was 2.18% compared to 1.97% obtained with the conventional method of fermentation and subsequent ethanol reflux extraction.

Key words: vanillin, cellulase, condition optimization