食品科学

• 工艺技术 • 上一篇    下一篇

超声波辅助提取常熟黑米类黄酮及其抗氧化活性分析

杨志刚,张燕萍,杨海定   

  1. 常熟理工学院生物与食品工程系,江苏 常熟 215500
  • 出版日期:2013-09-25 发布日期:2013-09-27

Ultrasonic-Assisted Extraction and Antioxidant Activities of Flavonids from Changshu Grown Black Rice

YANG Zhi-gang,ZHANG Yan-ping,YANG Hai-ding   

  1. Department of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
  • Online:2013-09-25 Published:2013-09-27

摘要:

以常熟黑米类黄酮的提取率为指标,采用单因素试验和正交试验对超声波辅助提取工艺条件进行优选,并通过测定常熟黑米类黄酮对DPPH自由基、羟自由基(•OH)、超氧阴离子自由基(O-2 •)的清除作用,研究其抗氧化活性。结果表明:常熟黑米类黄酮最佳提取工艺条件为料液比1:60(g/mL)、乙醇体积分数60%、超声功率288W、浸提温度50℃、浸提时间40min,在此条件下提取率为1.415%。常熟黑米类黄酮表现出较好的抗氧化活性,且与质量浓度呈正相关,但抗氧化能力小于VC,是一种很好的天然抗氧化剂。

关键词: 常熟黑米, 类黄酮, 超声波辅助提取, 抗氧化

Abstract:

The ultrasonic-assisted extraction of flavonoids from Changeshu grown black (duck blood) rice was
optimized by single-factor experiments and orthogonal experiment design to obtain maximum flavonoid yield.
The extracted flavnoids were investigated for their antioxidant activities by DPPH radical scavenging, hydroxyl
radical scavenging and superoxide anion radical scavenging assays. The results showed that the optimum
extraction conditions for solid-to-solvent ratio, ethanol concentration, ultrasonic power, temperature and
time were 1:60 (g/mL), 60%, 288 W, 50 ℃ and 40 min, respectively. The maximum yield of flavonoids under
these conditions was 1.415%. The flavonoids obtained exhibited powerful antioxidant activity in a concentrationdependent
manner, which, however, was inferior to that of VC. Therefore, this study concludes that Changshu black
rice is a good source of natural antioxidants.

Key words: Changshu black rice, flavonoids, ultrasonic-assisted extraction, antioxidant