食品科学

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冷藏期间草鱼肌肉脂质降解的影响因素分析

王建辉,刘冬敏,刘永乐,王发祥,俞 健,李向红,杨 晶   

  1. 长沙理工大学化学与生物工程学院,湖南 长沙 410114
  • 出版日期:2013-09-25 发布日期:2013-09-27

Factors Influencing Lipid Degradation of Grass Carp Muscle during Cold Storage

WANG Jian-hui,LIU Dong-min,LIU Yong-le,WANG Fa-xiang,YU Jian,LI Xiang-hong,YANG Jing   

  1. School of Chemistry and Bioengineering, Changsha University of Science and Technology, Changsha 410114, China
  • Online:2013-09-25 Published:2013-09-27

摘要:

分别以酸价(AV)、过氧化值(POV)、硫代巴比妥酸值(TBARs)作为脂肪的水解、初期氧化和终期氧化指标,结合脂肪酶和脂肪氧化酶活性的动态变化,研究冷藏(2~4℃)条件下草鱼肌肉脂肪的水解、氧化变化规律,探讨光照、脂肪酶及脂肪氧化酶对冷藏草鱼肌肉脂质的影响。结果表明:冷藏过程中,草鱼肌肉脂肪不断发生水解和氧化。避光组和光照组草鱼肌肉脂肪分别从第6天和第4天开始大量水解,肌肉内游离脂肪酸大量积累;脂肪水解的同时,脂肪酸开始氧化,避光组和光照组鱼肉中氢过氧化物大量积累,分别于冷藏的第6天和第8天达最大值;随着氧化进程的发展,体系TBARs值不断升高。光照显著增加了冷藏草鱼肌肉脂肪POV及TBARs值(P<0.05),脂肪酶、脂肪氧化酶明显加速了脂质的水解和氧化降解。冷藏条件下草鱼肌肉脂肪的水解和氧化加快,脂肪劣变程度加深。因此,对新鲜草鱼肌肉进行避光和酶钝化处理有利于减少冷藏期间草鱼肌肉脂质的降解,保证肌肉较高的营养价值及加工品质,延长其货架期。

关键词: 草鱼, 脂质, 氧化, 水解, 光照, 脂肪酶, 脂肪氧化酶

Abstract:

The effect of light, lipase and lipoxygenase on lipid oxidation in frozen grass carp muscle during cold
storage was investigated. Acid value (AV), peroxide value (POV) and thiobarbituric acid reactive substances
(TBARs) were measured as indicators of lipid hydrolysis and initial and final lipid oxidation. During cold storage,
the hydrolysis and oxidation of lipids in fish muscle continued. Lipid hydrolysis in large quantities began on the 6th
and 4th days of storage in the light and in the dark, respectively, leading to the accumulation of large amounts of free
fatty acids. This was accompanied by the occurrence of lipid oxidation and thus the accumulation of large amounts
of hydrogen peroxide. The peroxide value (POV) reached maximum during storage on day 6 in the dark and on day
8 in the light. The TBARs value increased as the lipid oxidation continued. The POV and TBARs value became
significantly higher during storage in the light (P < 0.05). Both lipase and lipoxygenase were able to considerably
accelerate the hydrolytic and oxidative degradation of lipids. In addition, this phenomenon and consequent lipid
deterioration were also observed during cold storage. Therefore, dark storage and enzyme inactivation can help
reduce the lipid oxidation in grass carp muscle during cold storage, maintain its high nutritive value and processing
quality and prolong its shelf life.

Key words: grass carp, fat, oxidation, hydrolysis, light, lipase, lipoxidase