食品科学

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冷榨葡萄籽饼粕超微粉对衰老小鼠内脏抗氧化功能的影响

刘 霞1,杨继红1,2,3,王海瑞4,隋银强1,李 华1,2,3,王 华1,2,3,*   

  1. 1.西北农林科技大学葡萄酒学院,陕西 杨凌 712100;2.陕西省葡萄与葡萄酒工程研究中心,陕西 杨凌 712100;
    3.西北农林科技大学葡萄与葡萄酒(合阳)试验站,陕西 合阳 715300;4.西安新药评价中心,陕西 西安 710065
  • 出版日期:2013-09-15 发布日期:2013-09-27
  • 通讯作者: 王 华
  • 基金资助:

    科技部农业科技成果转化基金资助项目(K304021001);国家2008年杨凌葡萄苗木良种繁育基地项目

Effect of Cold-pressed Grape Seed Superfine Powder (CP-GSSP) on Antioxidative Functions in Aging Mouse Viscera

LIU Xia1,YANG Ji-hong1,2,3,WANG Hai-rui4,SUI Yin-qiang1,LI Hua1,2,3,WANG Hua1,2,3,*   

  1. 1. College of Enology, Northwest A&F University, Yangling 712100, China;2. Shaanxi Engineering Research Center for
    Viti-viniculture, Yangling 712100, China;3. Viti-viniculture Experiment Station (Heyang) of Northwest A&F University,
    Heyang 715300, China;4. Xi’an New Drug Evaluation and Research Center, Xi’an 710065, China
  • Online:2013-09-15 Published:2013-09-27
  • Contact: WANG Hua

摘要:

目的:研究酿酒副产物葡萄籽冷榨提油后饼粕超微粉对衰老小鼠心脏、肾脏和肝脏的抗氧化功能的影响。方法:60只小鼠随机分为冷榨葡萄籽饼粕超微粉(CP-GSSP)低、中、高剂量组(323、646、1938mg/(kg·d))、VE 组(25mg/(kg·d))、衰老模型组和空白组。除空白组腹腔注射等体积生理盐水外,其他各组腹腔注射D-半乳糖构建亚急性衰老模型,同时剂量组灌胃给予CP-GSSP,VE组给予油溶性VE,模型组和空白组均给予同体积食用油。持续45d后,测定心脏、肾脏和肝脏中丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力。结果:形态水平:CP-GSSP可以抵抗由D-半乳糖所致的衰老表征的出现并且对内脏无不良影响。生化水平:CP-GSSP能够显著降低小鼠心脏、肾脏和肝脏中脂质过氧化物MDA的含量,提高抗氧化酶SOD和GSH-Px的活力。结论:CP-GSSP具有显著抑制衰老小鼠体内脂质过氧化的作用、能明显提高内源性抗氧化酶活性并且无毒副作用,表明其具有较好的抗氧化、抗衰老作用。

关键词: 冷榨, 葡萄籽, 超微粉, 内脏, 抗氧化

Abstract:

Objective: To explore the effect of cold-pressed grape seed superfine powder (CP-GSSP) on visceral anti-oxidative
function in aging mice. Methods: Sixty mice were randomly divided into CP-GSSP low, medium and high dose groups (323,
646, 1938 mg/(kg•d)), VE group (25 mg/(kg•d)), aging model group and normal control group. The mice in the normal control
group were intraperitoneally injected with physiological saline, whereas, other groups were intraperitoneally injected with
D-galactose to establish aging mouse model. Along with intraperitoneal injection, the mice from the CP-GSSP groups, VE
group, aging model group and normal control group were intragastrically administered with CP-GSSP at various doses, VE,
edible oil and edible oil, respectively. Forty-five days later, the content of MDA, and the activities of SOD and
GSH-Px in heart, kidney and liver were detected. Results: CP-GSSP resisted the aging performance caused by D-galactose
without causing adverse effects in viscera, and significantly reduced the content of MDA, and improved the activities of
SOD and GSH-Px in heart, kidney and liver. Conclusion: CP-GSSP can inhibit lipid peroxidation and improve the activity of
endogenous antioxidases in aging mice. Therefore, it has a good antioxidant and anti-aging effect.

Key words: cold-pressed grape seeds, superfine powder, viscera, antioxidation

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