食品科学

• 基础研究 • 上一篇    下一篇

草质素体外清除自由基及抑制蛋白质氧化的作用

乔 燕,刘学波*   

  1. 西北农林科技大学食品科学与工程学院,陕西 杨凌 712100
  • 出版日期:2013-09-15 发布日期:2013-09-27
  • 通讯作者: 刘学波

in vitro Free Radical Scavenging and Protein Oxidation Inhibitory Effects of Herbacetin

QIAO Yan,LIU Xue-bo*   

  1. College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
  • Online:2013-09-15 Published:2013-09-27
  • Contact: LIU Xue-bo

摘要:

从DPPH自由基、羟自由基(·OH)清除能力以及对Cu2+-H2O2/AAPH体系诱导蛋白氧化损伤及羰基化的抑制4个方面对草质素的体外抗氧化活性进行观察。结果表明:10~100μmol/L的草质素能够显著清除DPPH自由基和·OH,半抑制浓度(IC50值)分别为49.28、219.20μmol/L。同时,草质素呈浓度依赖方式抑制Cu2+-H2O2及AAPH诱导的蛋白氧化损伤及羰基化。草质素具有较强清除自由基和抑制蛋白质氧化的能力。

关键词: 草质素, 自由基, 蛋白损伤, 羰基化, 抗氧化

Abstract:

The in vitro antioxidant activity of herbacetin was investigated in terms of scavenging activities against DPPH
and hydroxyl free radicals and inhibitory effects against oxidative protein damage and carbonylation induced by Cu2+-
H2O2 or AAPH. At a concentration between 10 μmol/L and 100 μmol/L, herbacetin exerted a marked scavenging effect on
DPPH and hydroxyl free radicals, with respective IC50 values of 49.28 μmol/L and 219.2 μmol/L. Meanwhile, herbacetin could
significantly inhibit oxidative protein damage and carbonylation induced by Cu2+-H2O2 or AAPH in a dose-dependent manner.
This study concludes that herbacetin has a strong ability to scavenge free radical and inhibit oxidative protein damage.

Key words: herbacetin, free radical, protein damage, carbonylation, antioxidant activity

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