食品科学

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一株产γ-氨基丁酸植物乳杆菌MJ0301培养基的优化

黄桂东1,毛 健1,2,*,姬中伟1,傅健伟2,邹慧君2   

  1. 1.江南大学食品学院,江苏 无锡 214122;2.国家黄酒工程技术研究中心,浙江 绍兴 312000
  • 出版日期:2013-09-15 发布日期:2013-09-27
  • 通讯作者: 毛 健
  • 基金资助:

    江南大学自主科研计划项目(1022050205123950)

Optimization of Culture Medium Formulation for γ-Aminobutyric Acid-producing Lactobacillus plantarum MJ0301

HUANG Gui-dong1,MAO Jian1,2,*,JI Zhong-wei1,FU Jian-wei2,ZOU Hui-jun2   

  1. 1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
    2. National Engineering Research Center of Chinese Rice Wine, Shaoxing 312000, China
  • Online:2013-09-15 Published:2013-09-27
  • Contact: MAO Jian

摘要:

对一株具有产γ-氨基丁酸(γ-aminobutyric acid,GABA)能力的植物乳杆菌(Lactobacillus plantarum MJ0301)进行发酵培养基成分优化,以提高该菌产GABA能力。通过单因素试验考察碳源、氮源、K2HPO4、吐温-80和L-谷氨酸钠(monosodium L-glutamate,L-MSG)对L.plantarum MJ0301生长及GABA产量的影响。结果表明,葡萄糖、氮源(胰蛋白胨和酵母膏)和L-谷氨酸钠3个因素对L. plantarum MJ0301生长和GABA产量影响较大。采用Box-Behnken中心组合试验设计原理,选定葡萄糖、复合氮源(胰蛋白胨和酵母膏)和L-谷氨酸钠为三因素三水平进行响应面试验,以GABA产量为响应值,在分析各个因素的显著性和交互作用后,确定L. plantarum MJ0301产GABA的最佳培养基成分,即葡萄糖16.0g/L、氮源10g/L、L-谷氨酸钠30g/L、柠檬酸三胺2g/L、K2HPO4 2g/L、乙酸钠5g/L、硫酸镁0.1g/L、硫酸锰0.05g/L、吐温-80 1mL/L。此时,L. plantarum MJ0301产GABA的量由原来的143mg/L增加到1.59g/L,提高了10.12倍。

关键词: 植物乳杆菌MJ0301, &gamma, -氨基丁酸, 响应面分析法

Abstract:

The aim of the present study was to investigate the optimization of formulation of culture medium for γ-aminobutyric
acid (GABA)-producing lactic acid bacteria. The effects of carbon sources, nitrogen sources, K2HPO4, Tween-80 and monosodium
L-glutamate (L-MSG) on the growth of L. plantarum MJ0301 and the yield of GABA were explored by single factor test. The
results showed that glucose, nitrogen (tryptone and yeast extract) and L-MSG significantly influenced the growth of L. plantarum
MJ0301 and the yield of GABA (P < 0.05). The response surface methodology was designed based on glucose, nitrogen (tryptone
and yeast extract) and L-MSG, and the yield of GABA was considered as response value. The optimal formulation of the culture
medium consisted of glucose 16.0 g/L, mixed nitrogen sources 10 g/L, L-MSG 30 g/L, ammonium citrate 2 g/L, K2HPO4
2 g/L, sodium acetate 5 g/L, magnesium sulfate 0.1 g/L, manganese sulfate 0.05 g/L, and Tween-80 1 mL/L. The resulting yield of
GABA was 1.59 g/L, which was 11.12-fold higher than before the optimization (143 mg/L).

Key words: Lactobacillus plantarum MJ0301;&gamma, -aminobutyric acid;response surface methodology

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