食品科学

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Eu-苦瓜多糖复合物的合成、抑制食道癌细胞增殖及与DNA相互作用

张 翠1,2,孙晓波1,宋爱荣2,潘 维1,韩志建1,2,丁鲁军1,王进平1,2,*   

  1. 1.青岛农业大学化学与药学院,山东 青岛 266109;
    2.青岛农业大学 山东省应用真菌重点实验室,山东 青岛 266109
  • 出版日期:2013-09-15 发布日期:2013-09-27
  • 通讯作者: 王进平

Synthesis and Antiproliferative Effect on Esophagus Cancer Cells and Interaction with DNA of Eu-Momordica charantia L. Polysaccharide Complex

ZHANG Cui1,2,SUN Xiao-bo1,SONG Ai-rong2,PAN Wei1,HAN Zhi-jian1,2,DING Lu-jun1,WANG Jin-ping1,2,*   

  1. 1. College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China;
    2. Shandong Provincial Key Laboratory of Applied Mycology, Qingdao Agricultural University, Qingdao 266109, China
  • Online:2013-09-15 Published:2013-09-27
  • Contact: WANG Jin-ping

摘要:

目的:利用多糖提高机体免疫功能的抗肿瘤机理和稀土直接杀死、杀伤肿瘤细胞的药用价值,合成稀土-多糖复合物,发挥二者的协同作用,开发具有抗肿瘤作用的食品或保健品。方法:以苦瓜多糖为模板,均匀沉淀法制备稀土(Eu)-苦瓜多糖复合物(Eu-MCPS);利用红外光谱、扫描电镜和透射电镜对其进行结构表征;噻唑蓝(MTT)比色法测定复合物对食道癌细胞的体外增殖抑制作用;光谱法研究Eu-MCPS与鲱鱼精脱氧核糖核酸(DNA)的相互作用。结果:稀土(Eu)以Eu(OH)CO3颗粒的形式附着在相互缠绕螺旋的多糖链上,并不通过化学键连接,Eu-MCPS形成,粒径分布在340~540nm。Eu-MCPS的质量浓度为100μg/mL时,对食管癌细胞的生长抑制率可达到21.1%,表明稀土-苦瓜多糖复合物对食管癌细胞具有一定的体外增殖抑制作用,并且光谱法证明稀土-苦瓜多糖复合物与DNA发生非嵌插作用。

关键词: 多糖, 稀土, 稀土-苦瓜多糖复合物微粒, 抗肿瘤

Abstract:

Objective: Polysaccharides have antitumor activity due to the stimulation to immune functions of human
body, while rare earth elements have antitumor ability by killing tumor cells. In order to exploit food or healthcare
products with antitumor effect, Eu-Momordica charantia L. polysaccharide complex (Eu-MCPS) was synthesized
with the expectation of synergistic antitumor effects of the individuals. Methods: Eu-MCPS was synthesized with
homogeneous precipitation by using Momordica charantia L. polysaccharide as the template. The complex was
subjected to structural characterization employing FT-IR, scanning electron microscope and transmission electron
microscope and its antitumor activity was evaluated by MTT assay. In addition, the interaction between Eu-MCPS
and DNA was studied by UV-Vis spectroscopy and fluorescence spectroscopy. Results: Eu adhered to the twining
and helical chains of Momordica charantia L. polysaccharide in the form of Eu(OH)CO3 particles instead of forming
chemical bond with it. The particle size of Eu-MCPS varied from 340 nm to 540 nm. Eu-MCPS inhibited the
proliferation of esophagus cancer cells, and its inhibitory rate was 21.1% at the concentration of 100 μg/mL. The
interaction between Eu-MCPS and DNA may be a non-intercalation mode. These results can provide useful references
to develop special functional foods or healthcare products.

Key words: polysaccharide, rare earth metal, Eu-MCPS, antitumor activity

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