响应面法优化梅花鹿胎盘多肽超声波杀菌工艺

doi:10.7506/spkx1002-6630-201404009

食品科学 ›› 2014, Vol. 35 ›› Issue (4): 39-43.doi: 10.7506/spkx1002-6630-201404009

响应面法优化梅花鹿胎盘多肽超声波杀菌工艺

皮钰珍，关楚谕，孙文华，岳喜庆

沈阳农业大学食品学院，辽宁沈阳 110086

收稿日期:2013-06-17 修回日期:2014-02-17 出版日期:2014-02-25 发布日期:2014-03-17
通讯作者: 皮钰珍 E-mail:1014436061@qq.com
基金资助:
沈阳市科学技术计划项目（F12-120-3-00）

Optimization of Ultrasonic Sterilization Process of Sika Deer Placenta Polypeptides by Response Surface Methodology

PI Yu-zhen, GUAN Chu-yu, SUN Wen-hua, YUE Xi-qing

College of Food Science, Shenyang Agricultural University, Shenyang 110086, China

Received:2013-06-17 Revised:2014-02-17 Online:2014-02-25 Published:2014-03-17
Contact: PI Yu-zhen E-mail:1014436061@qq.com

摘要/Abstract

摘要：

以酶解获得的梅花鹿胎盘多肽为原料，进行超声波杀菌，研究超声功率、超声时间和脉冲时间对多肽杀菌率的影响。利用响应面分析法确定了超声波杀菌的最佳工艺参数为：超声功率850 W、超声时间4.6 min、脉冲时间7 s，此时梅花鹿胎盘多肽的损失率为20.2%，多肽的杀菌率为87.5%，其中细菌总数为68 CFU/mL，大肠杆菌最大可能数为零，均符合国家标准。在最佳杀菌工艺条件下获得的梅花鹿胎盘多肽，对超氧阴离子自由基的清除率比未经杀菌处理的多肽仅降低了3.3%，对羟自由基的清除率降低了1.1%，对DPPH自由基的清除率降低了1.7%。这说明超声波杀菌后的梅花鹿胎盘多肽既达到了商业无菌的标准，又有效地保持了其生物活性。

关键词: 梅花鹿胎盘多肽, 超声波杀菌, 杀菌率

Abstract:

In this experiment, polypeptides obtained from enzymatic hydrolysis of sika deer placenta were sterilized by
ultrasonic radiation. The effects of ultrasonic intensity, treatment time and impulse time on sterilization efficiency were
investigated. The optimal parameters were determined by response surface methodology as follows: 850 W of ultrasonic
intensity, 4.6 min of ultrasonic treatment time and 7 s of ultrasonic impulse time. The results showed that the polypeptide
loss rate was 20.2% and the sterilization efficiency was 87.5% under these conditions. In addition, the total bacterial count
was 68 CFU/mL and the most probable number (MPN) of Escherichia coli was zero. All these parameters complied with the
national standards. The free radical scavenging activity of sterilized sika deer placenta polypeptides against superoxide anion,
hydrolxyl and DPPH radicals was decreased by only 3.3%, 1.1% and 1.7% as compared to the unsterilized counterpart.
These results indicate that sterilized sika deer placenta polypeptides reach the requirements of commercial sterilization while
maintaining their bioactivity.

Key words: sika deer placenta polypeptides, ultrasonic sterilization, sterilization efficiency

中图分类号:

TS251.95

皮钰珍，关楚谕，孙文华，岳喜庆. 响应面法优化梅花鹿胎盘多肽超声波杀菌工艺[J]. 食品科学, 2014, 35(4): 39-43.

PI Yu-zhen, GUAN Chu-yu, SUN Wen-hua, YUE Xi-qing. Optimization of Ultrasonic Sterilization Process of Sika Deer Placenta Polypeptides by Response Surface Methodology[J]. FOOD SCIENCE, 2014, 35(4): 39-43.

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响应面法优化梅花鹿胎盘多肽超声波杀菌工艺

Optimization of Ultrasonic Sterilization Process of Sika Deer Placenta Polypeptides by Response Surface Methodology

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