大粒车前子多糖酸水解产物的分析

doi:10.7506/spkx1002-6630-201406012

食品科学 ›› 2014, Vol. 35 ›› Issue (6): 60-64.doi: 10.7506/spkx1002-6630-201406012

大粒车前子多糖酸水解产物的分析

胡海涛，王远兴*，张娟，邓静

南昌大学食品科学与技术国家重点实验室，江西南昌 330047

收稿日期:2013-06-28 修回日期:2014-03-11 出版日期:2014-03-25 发布日期:2014-04-04
通讯作者: 王远兴 E-mail:yuanxingwang@163.com
基金资助:
国家自然科学基金地区科学基金项目（31160321）

Product Analysis of Plantago asiatica L. Polysaccharide by Acid Hydrolysis

HU Hai-tao, WANG Yuan-xing*, ZHANG Juan, DENG Jing

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Received:2013-06-28 Revised:2014-03-11 Online:2014-03-25 Published:2014-04-04
Contact: WANG Yuan-xing E-mail:yuanxingwang@163.com

摘要/Abstract

摘要：

经水提、醇沉、Sevag除蛋白后得到精制大粒车前子多糖（Plantago asiatica L. polysaccharide，PLP），采用0.3 mol/L三氟乙酸对PLP进行部分酸水解。建立超高效液相色谱-蒸发光散射检测器技术筛选较优水解条件的方法，同时运用超高效液相色谱-四极杆飞行时间质谱联用法分析产物。结果表明：车前子多糖除蛋白后多糖含量为68.56%。在水解温度90 ℃、水解时间1.5 h条件下，能够得到比较多的水解产物，经超高效液相色谱-四极杆飞行时间质谱联用分析鉴定，水解产物中含有单糖、二糖至低聚六糖，并且存在戊糖和己糖相互连接的片段，但并未发现多个己糖相互连接的片段。

关键词: 大粒车前子, 部分酸水解, 超高效液相色谱-蒸发光散射检测器, 超高效液相色谱-四极杆飞行时间质谱联用

Abstract:

Polysaccharides were isolated from seeds of Plantago asiatica L. by water extraction and ethanol precipitation, and
subjected to protein removal by Sevag method, and then the polysaccharide sample was hydrolyzed with 0.3 mol/L TFA by partial
acid hydrolysis for different durations. An ultra performance liquid chromatography coupled with evaporative light scattering
detection (UPLC-ELSD) method was established for establishing better hydrolysis conditions, and ultra performance liquid
chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used for product analysis
of samples under the optimal hydrolysis conditions. Results showed that the content of polysaccharides in the deproteinized
sample was 68.56%. The optimal hydrolysis conditions were found to be hydrolysis with 0.3 mol/L TFA at 90 ℃ for 1.5 h. The
hydrolysate obtained under these conditions contained more degradation products like monosaccharides and oligosaccharides,
as identified by UPLC-Q-TOF-MS. Simultaneously, some pentose and hexose interconnected pieces in branch chains were also
detected. However, multiple hexose connected pieces were not observed.

Key words: Plantago asiatica L., partial acid hydrolysis, ultra performance liquid chromatography-evaporative lightscattering detector, ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry

中图分类号:

TS207.3

胡海涛，王远兴*，张娟，邓静. 大粒车前子多糖酸水解产物的分析[J]. 食品科学, 2014, 35(6): 60-64.

HU Hai-tao, WANG Yuan-xing*, ZHANG Juan, DENG Jing. Product Analysis of Plantago asiatica L. Polysaccharide by Acid Hydrolysis[J]. FOOD SCIENCE, 2014, 35(6): 60-64.

参考文献

[1] 中华人民共和国卫生部药典委员会. 中华人民共和国药典: 一部[M].北京: 化学工业出版社, 2005: 46

[2] 王隶书, 李明洋, 宋柏林, 等. 车前子多糖研究进展[J]. 时珍国医国药, 2012, 23(2): 460-462.

[3] 殷军艺, 聂少平, 林慧霞, 等. 大粒车前子多糖乙醇分级及其理化性质研究[J]. 食品科学, 2009, 30(17): 34-37.

[4] ROMERO A L, WEST K L, ZEM T, et al. The seeds from Plantagoovata lower plasma lipids by altering hepatic and bile acid metabolism in guinea pigs[J]. The Journal of Nutrition, 2002, 132: 1194-1198.

[5] FANG Chenshun. Dietary Psyllium reverses hypercholesterolemic effect of trans fatty in rats[J]. Nutrition Research, 2000, 20(5): 695-705.

[6] Stanisavljevi? I T, Stoji?evi? S S, Veli?kovi? D T, et al. Screening the antioxidant and antimicrobial properties of the extracts from plantain (Plantago major L.) leaves[J]. Separation Science and Technology, 2008, 43(14): 3652-3662.

[7] 林慧霞, 聂少平, 殷军艺, 等. 大粒车前子多糖提取工艺优化及其理化性质测定[J]. 食品科学, 2010, 31(22): 226-231.

[8] 周超, 谢明勇, 万茵, 等. 车前子多糖的测定方法研究[J]. 分析试验室, 2008, 27(4): 10-13.

[9] Yin J, Lin H, Li J, et al. Structural characterization of a highly branched polysaccharide from the seeds of Plantago asiatica L[J]. Carbohydrate Polymers, 2012, 87(4): 2416-2424.

[10] Yin J Y, Lin H X, Nie S P, et al. Methylation and 2D NMR analysis of arabinoxylan from the seeds of Plantago asiatica L[J]. Carbohydrate Polymers, 2012.

[11] Yin J Y, Nie S P, Zhou C, et al. Chemical characteristics and antioxidant activities of polysaccharide purified from the seeds of Plantago asiatica L[J]. Journal of the Science of Food and Agriculture, 2010, 90(2): 210-217.

[12] Huang D F, Tang Y F, Nie S P, et al. Effect of phenylethanoid glycosides and polysaccharides from the seed of Plantago asiatica L. on the maturation of murine bone marrow-derived dendritic cells[J]. European journal of pharmacology, 2009, 620(1): 105-111.

[13] Huang D F, Xie M Y, Yin J Y, et al. Immunomodulatory activity of the seeds of Plantago asiatica L[J]. Journal of ethnopharmacology, 2009, 124(3): 493-498.

[14] Guan J, Li S P. Discrimination of polysaccharides from traditional Chinese medicines using saccharide mapping—Enzymatic digestion followed by chromatographic analysis[J]. Journal of pharmaceutical and biomedical analysis, 2010, 51(3): 590-598.

[15] Xu J, Guan J, Chen X J, et al. Comparison of polysaccharides from different Dendrobium using saccharide mapping[J]. Journal of pharmaceutical and biomedical analysis, 2011, 55(5): 977-983.

[16] Guan J, Zhao J, Feng K, et al. Comparison and characterization of polysaccharides from natural and cultured Cordyceps using saccharide mapping[J]. Analytical and bioanalytical chemistry, 2011, 399(10): 3465-3474.

[17] 周超, 谢明勇, 万茵, 等. 车前子多糖的测定方法研究[J]. 分析试验室, 2008, 27(4): 10-13.

[18] 张媛媛, 聂少平, 万成, 等. 高效液相色谱-蒸发光散射检测法同时测定单糖, 双糖及低聚果糖[J]. 食品科学, 2009, 30(18): 237-239.

[19] 殷军艺, 林慧霞, 李菁, 等. 大粒车前子多糖 PLP-2 结构研究[J].

[20] 孙元琳, 申瑞玲, 汤坚, 等. 当归多糖的水解特征及其水解产物分析[J]. 分析化学 (FENXI HUAXUE), 2008, 36.

[21] Gao Q, Nilsson U, Ilag L L, et al. Monosaccharide compositional analysis of marine polysaccharides by hydrophilic interaction liquid chromatography-tandem mass spectrometry[J]. Analytical and bioanalytical chemistry, 2011, 399(7): 2517-2529.

[22] 殷军艺, 聂少平, 付志红, 等. 大粒车前子多糖分离, 纯化及单糖组成分析[J]. 食品科学, 2008, 29(9): 529-32.

大粒车前子多糖酸水解产物的分析

Product Analysis of Plantago asiatica L. Polysaccharide by Acid Hydrolysis

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