葡萄籽原花青素对顺铂诱导人胚肾细胞凋亡的拮抗作用

doi:10.7506/spkx1002-6630-201401046

食品科学 ›› 2014, Vol. 35 ›› Issue (1): 234-238.doi: 10.7506/spkx1002-6630-201401046

葡萄籽原花青素对顺铂诱导人胚肾细胞凋亡的拮抗作用

郭卓雨，高丽萍*，李贞

北京联合大学应用文理学院，生物活性物质与功能食品北京市重点实验室，北京 100191

收稿日期:2013-06-29 修回日期:2013-12-24 出版日期:2014-01-15 发布日期:2014-01-22
通讯作者: 高丽萍 E-mail:gaolip62@163.com
基金资助:
北京联合大学人才强校计划人才资助项目（BPHR2011A01）

Protective Effect of Grape Seed Proanthocyanidin Extract against Cisplatin-Induced Apoptosis in Human Embryonic Kidney Cells

GUO Zhuo-yu, GAO Li-ping*, LI Zhen

Beijing Municipal Key Laboratory of Biological Active Substance and Functional Food, College of Applied Arts and Technology,
Beijing Union University, Beijing 100191, China

Received:2013-06-29 Revised:2013-12-24 Online:2014-01-15 Published:2014-01-22
Contact: GAO Li-ping E-mail:gaolip62@163.com

摘要/Abstract

摘要：

目的：探讨葡萄籽原花青素（ g r a p e s e e d p r o a n t h o c y a n i d i n ， G S P E ）对顺铂（cis-diamminedichloroplatinum，CDDP）诱导人胚肾细胞HEK293凋亡及相关凋亡基因的影响，并探讨可能的机制。方法：体外培养正常HEK293细胞，噻唑蓝法测定GSPE、CDDP分别对HEK293细胞生长的影响以及GSPE对CDDP诱导HEK293细胞毒性的保护作用，流式细胞仪测定GSPE对CDDP所致HEK293细胞凋亡率的变化，Western blotting测定GSPE对CDDP所致HEK293细胞凋亡相关基因Bax、Bcl-2蛋白表达的影响。结果：GSPE对CDDP所致HEK293细胞毒性具有拮抗作用，可减轻CDDP所致HEK293细胞凋亡，减弱Bax表达、增强Bcl-2表达。结论：GSPE对CDDP所致HEK293细胞凋亡具有拮抗作用，其机制可能与GSPE降低促凋亡基因Bax，升高抗凋亡基因Bcl-2的表达有关。

关键词: 葡萄籽原花青素, 顺铂, 人胚肾细胞HEK293, 细胞凋亡

Abstract:

Purpose: To explore the protective effect of grape seed proanthocyanidin extract (GSPE) against cisplatin
(CDDP)-induced apoptosis in human embryo kidney (HEK) 293 cells and its possible mechanisms. Methods: The effects of
CDDP and GSPE on the in vitro growth of HEK 293 cells and the effect of GSPE on CDDP-induced toxicity was evaluated
by MTT assay. CDDP-induced apoptosis in HEK293 cells was analyzed by flow cytometry. Western blotting was applied
to determine the expression of apoptotic gene Bax and anti-apoptosis gene Bcl-2. Results: GSPE was able to antagonize
cisplatin-induced cytotoxicity in a concentration-dependent manner. Flow cytometry revealed that GSPE could lead to a
decrease in apoptosis rate and pro-apoptotic Bax gene expression while anti-apoptotic gene Bcl-2 expression was enhanced.
Conclusion: GSPE demonstrates a preventive effect on cisplatin-induced injury in HEK293 cells. The mechanism may be
related to its capacity of enhancing anti-apoptotic gene Bcl-2 expression and inhibiting pro-apoptotic gene Bax expression.

Key words: grape seed proanthocyanidin extract, cisplatin, human embryo kidney (HEK) 293 cells, apoptosis

中图分类号:

Q946.8

郭卓雨，高丽萍*，李贞. 葡萄籽原花青素对顺铂诱导人胚肾细胞凋亡的拮抗作用[J]. 食品科学, 2014, 35(1): 234-238.

GUO Zhuo-yu, GAO Li-ping*, LI Zhen. Protective Effect of Grape Seed Proanthocyanidin Extract against Cisplatin-Induced Apoptosis in Human Embryonic Kidney Cells[J]. FOOD SCIENCE, 2014, 35(1): 234-238.

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葡萄籽原花青素对顺铂诱导人胚肾细胞凋亡的拮抗作用

Protective Effect of Grape Seed Proanthocyanidin Extract against Cisplatin-Induced Apoptosis in Human Embryonic Kidney Cells

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