关键词: 高效液相色谱-串联质谱法, 有机磷农药, 残留, 香菇

Abstract:

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS-MS) method was established
for the determination of 23 organophosphorus pesticide residues in Lentinus edodes. Samples were extracted with acetonitrile
and separated by salting out method, and then the supernatants were purified using dispersive solid-phase extraction (C18 and
primary-secondary amine powder) prior to HPLC-MS-MS analysis. The analytes were identified in positive electrospray
ionization and multiple reaction-monitoring modes. The matrix-matched external standard calibration curves were used
for quantitative analysis. Under the optimal conditions, the limit of quantitation of the method was 0.01 mg/kg. The
recoveries of 23 organophosphorus pesticides in spiked real samples were 70.2%–105.0% with relative standard deviation
of 3.5%–13.0%. The method presented is sensitive, accurate and fast and can meet the requirements for the determination of
organophosphorus pesticide residues in Lentinus edodes.

Key words: high performance liquid chromatography-tandem mass spectrometry, organophosphorus pesticide, residues, Lentinus edodes