绿原酸缓解肠道线粒体损伤研究

doi:10.7506/spkx1002-6630-201321070

食品科学 ›› 2013, Vol. 34 ›› Issue (21): 349-353.doi: 10.7506/spkx1002-6630-201321070

绿原酸缓解肠道线粒体损伤研究

周丽丽，阮征*，周艳，杨玉辉，李小兰，江敏

南昌大学生命科学与食品工程学院，食品科学与技术国家重点实验室，江西南昌 330047

收稿日期:2013-06-15 修回日期:2013-10-11 出版日期:2013-11-15 发布日期:2013-10-28
通讯作者: 阮征 E-mail:ruanzheng@ncu.edu.cn

Effect of Chlorogenic Acid on Damaged Intestinal Mitochondria in Rats

ZHOU Li-li，RUAN Zheng*，ZHOU Yan，YANG Yu-hui，LI Xiao-lan， JIANG Min

State Key Laboratory of Food Science and Technology, College of Life Science and Food Engineering, Nanchang University, Nanchang 330047, China

Received:2013-06-15 Revised:2013-10-11 Online:2013-11-15 Published:2013-10-28
Contact: Zheng RUAN E-mail:ruanzheng@ncu.edu.cn

摘要/Abstract

摘要：

目的：研究绿原酸对大鼠肠道线粒体损伤的缓解作用。方法：采用H2O2诱导大鼠肠道线粒体氧化损伤，通过测定线粒体肿胀度、膜电位、丙二醛(MDA)含量、总超氧化物歧化酶(T-SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活力，以及线粒体呼吸链复合物Ⅰ、Ⅳ、Ⅴ的活性，分析绿原酸对肠道线粒体损伤的缓解效果。结果：绿原酸可显著缓解H2O2造成的线粒体肿胀和膜电位的降低。绿原酸(0.032mg/mL和0.064mg/mL)显著降低MDA的含量，绿原酸(0.064mg/mL)能显著提高T-SOD、GSH-Px及复合物Ⅰ酶的活力。结论：绿原酸通过提高线粒体呼吸链复合物酶和抗氧化酶的活力，缓解线粒体H2O2的损伤。

关键词: 绿原酸, 线粒体, 氧化损伤, 呼吸链复合物

Abstract:

Objective: To explore if chlorogenic acid (CHA) could ameliorate intestinal mitochondrial damage in rats. Methods:
Intestinal mitochondria of rats were treated with hydrogen peroxide. The mitochondrial swelling, membrane potential, MDA content,
T-SOD and GSH-Px activities, and the enzymatic activities of mitochondrial respiratory chain complexes Ⅰ, Ⅳ, and Ⅴ were
evaluated. Results: CHA significantly inhibited mitochondrial swelling caused by hydrogen peroxide and reduced cell membrane
potential. CHA (0.032 mg/mL and 0.064 mg/mL) significantly decreased the concentration of MDA when compared with the control
group. The activities of T-SOD, GSH-Px and complex Ⅰ in the CHA group (0.064 mg/mL) were significantly increased when
compared with the control group. Conclusion: CHA can attenuate mitochondrial damage induced by hydrogen peroxide through
increasing the activities of mitochondrial respiratory chain complex enzymes and antioxidant enzymes.

Key words: chlorogenic acid, mitochondria, oxidative damage, respiratory chain complex

中图分类号:

TS201.4

周丽丽，阮征*，周艳，杨玉辉，李小兰，江敏. 绿原酸缓解肠道线粒体损伤研究[J]. 食品科学, 2013, 34(21): 349-353.

ZHOU Li-li，RUAN Zheng*，ZHOU Yan，YANG Yu-hui，LI Xiao-lan， JIANG Min. Effect of Chlorogenic Acid on Damaged Intestinal Mitochondria in Rats[J]. FOOD SCIENCE, 2013, 34(21): 349-353.

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绿原酸缓解肠道线粒体损伤研究

Effect of Chlorogenic Acid on Damaged Intestinal Mitochondria in Rats

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