食品科学

• 工艺技术 • 上一篇    下一篇

余甘子核仁油的微波辅助萃取工艺优化及脂肪酸组成分析

张雯雯,张 弘,郑 华,李 坤,冯 颖,甘 瑾,侯 彬,李成强   

  1. 1.中国林业科学研究院资源昆虫研究所,云南 昆明 650224;2.西南林业大学林学院,云南 昆明 650224
  • 出版日期:2013-10-25 发布日期:2013-09-28

Microwave-Assisted Extraction and Fatty Acid Composition Analysis of Oil from Phyllanthus emblica L. Seeds

ZHANG Wen-wen,ZHANG Hong,ZHENG Hua,LI Kun,FENG Ying,GAN Jin,HOU Bin,LI Cheng-qiang   

  1. 1. Research Institute of Resources Insects, Chinese Academy of Forestry, Kunming 650224, China;
    2. College of Forestry, Southwest Forestry University, Kunming 650224, China
  • Online:2013-10-25 Published:2013-09-28

摘要:

利用微波辅助提取余甘子核仁油,首先采用单因素试验对料液比、微波功率、微波时间进行考察,然后以油脂得率为响应值进行响应面优化,建立余甘子核仁油得率与各影响因素之间的数学模型。结果表明:余甘子核仁油的最佳提取工艺条件为料液比1:9.8(g/mL)、微波功率335W、微波时间12.3min,所得余甘子核仁油的得率为17.08%,提取级数为2级时油脂得率为18.76%。以气相色谱-质谱联用仪测定油脂的脂肪酸组成,检测结果表明,余甘子核仁油中不饱和脂肪酸占80.36%,其中亚麻酸占检出化合物的79.79%,此外还含有少量的棕榈油酸、11-二十碳烯酸(顺)以及8种饱和脂肪酸,且还检出了少量菜油甾醇、β-谷甾醇和VE,从余甘子核仁油的脂肪酸组成上来看,其营养价值较高。

关键词: 微波萃取, 余甘子, 气相色谱-质谱联用, 脂肪酸

Abstract:

The microwave-assisted extraction of seed oil from Phyllanthus emblica L. was optimized employing response
surface methodology. A mathematical model indicating the effect of solid/solvent ratio, microwave power and extraction
duration on oil yield was established. The optimal extraction conditions were found to be 1:9.8 (g/mL) of solid/solvent ratio,
335 W and 12.3 min. Under the optimized conditions, the yield of seed oil from Phyllanthus emblica L. was 17.08% and
increased to 18.76% when the extraction process was repeated once again. GC-MS analysis indicated that the major fatty
acids in the oil were unsaturated fatty acids, together accounting for 80.36% of the total lipids. Linolenic acid accounted for
79.79% of all the identified compounds. A small amount of palmitoleic acid, cis-11-eicosenoic acid and 8 kinds of saturated
fatty acids were also detected. Furthermore a small amount of campesterol, β-sitosterol and vitamin E were also detected.
The fatty acid composition showed the high nutritional value of the oil from Phyllanthus emblica L. seeds.

Key words: microwave-assisted extraction, Phyllanthus emblica L., gas chromatography-mass spectrometry (GC-MS), fatty acid