食品科学

• 工艺技术 • 上一篇    下一篇

高压均质降解壳聚糖对橘子汁澄清效果的影响

章 斌,侯小桢,凡芳华   

  1. 韩山师范学院生物系,广东 潮州 521041
  • 出版日期:2013-10-25 发布日期:2013-09-28

Influence of Chitosan Degradation by High Pressure Homogenization on Its Effect in Orange Juice Clarification

ZHANG Bin,HOU Xiao-zhen,FAN Fang-hua   

  1. Department of Biology, Hanshan Normal College, Chaozhou 521041, China
  • Online:2013-10-25 Published:2013-09-28

摘要:

为提高壳聚糖澄清橘子汁的效果,将其经过高压均质降解后,以透光率为指标,以未经降解的壳聚糖澄清效果为对照,探讨经高压均质降解的壳聚糖在不同均质压力、均质温度、均质时间和pH值条件下对橘子汁澄清效果的影响,并采用Box-Behnken响应面法进行40℃条件下壳聚糖高压均质工艺条件对澄清效果的响应面优化。结果表明:降解后的壳聚糖对橘子汁有较好的澄清效果,且明显优于未降解的对照组;建立的响应面模型能较好地预测透光率随高压均质处理条件的变化规律,其最优高压均质处理条件为壳聚糖用量0.723g/L、均质压力32MPa、均质时间6.8min、pH3.27,在此条件下按壳聚糖降解液:橘子原汁1:2.5比例添加降解液,可使橘子汁的透光率达到96.68%,使复原后的橘子原汁透光率达到92.2%。

关键词: 高压均质, 壳聚糖, 降解, 橘子汁, 澄清

Abstract:

Chitosan was degraded by high pressure homogenization to increase its effectiveness for clarifying
orange juice. The influence of homogenization pressure, temperature, time and pH on clarification of orange juice by
chitosan degradation products was examined by measuring the light transmittance of orange juice. The conditions for
clarifying orange juice at 40 ℃ were optimized using Box-Behnken experimental design combined with response surface
methodology. Results showed that degraded chitosan had obviously preferable clarification effect on orange juice when
compared to the control without degradation. Meanwhile, the response surface model allowed good prediction of juice
transmittance as a function of various homogenization conditions. Homogenization of 0.723 g/L acidic aquesou solution of
chitosan at pH 3.27 for 6.8 min at 32 MPa was found to be optimal. Transmittance was 96.68% for original orange juice and
92.2% for reconstituted orange juice when mixed with the degraded chitosan in a proportion of 1:2.5.

Key words: high pressure homogenization, chitosan, degradation, orange juice, clarification