实时荧光PCR法鉴定食品中鲑亚科鱼成分

doi:10.7506/spkx1002-6630-201320041

食品科学

实时荧光PCR法鉴定食品中鲑亚科鱼成分

李进波，李想，谌鸿超，潘良文，李正，宋青

1.华东理工大学生物工程学院，上海 200237；2.上海出入境检验检疫局，上海 200135

出版日期:2013-10-25 发布日期:2013-09-28

Identification of Salmoninae in Foods by Real-Time PCR

LI Jin-bo，LI Xiang，CHEN Hong-chao，PAN Liang-wen，LI Zheng，SONG Qing

1. School of Biotechnology, East China University of Science and Technology, Shanghai 200237, China；
2. Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China

Online:2013-10-25 Published:2013-09-28

摘要/Abstract

摘要：

根据鲑亚科鱼类生长激素基因保守序列设计特异性引物和探针，建立了一种鉴定食品中鲑亚科鱼类成分的实时荧光聚合酶链式反应(PCR)方法。结果表明：建立的检测方法高度特异于鲑亚科鱼类检测，鲑科的另两个亚科——白鲑亚科和茴鱼亚科样品均无扩增曲线。采用5种常见鲑亚科鱼品种可稳定检测到的最低DNA量为100pg；分别将大西洋鲑鱼和北极红点鲑鱼肉混入玉米、鸡肉和鲫鱼样品中测定的相对灵敏度为0.01%(m/m)。检测方法的重复性测试结果表明，Ct值标准偏差和相对标准偏差均在可接受范围内。运用建立的实时荧光PCR检测方法对25份市售实际样品进行测试，有4份标识含有“三文鱼”的样品未检测出鲑亚科鱼类成分。

关键词: 实时荧光聚合酶链式反应, 鲑亚科, 生长激素基因, 鉴定

Abstract:

A real-time PCR method was developed to indentify the ingredients of Salmoninae in foods. Specific primers
and probes were designed according to the conserved region of the growth hormone gene of Salmoninae. Results showed
that the developed method was highly specific for Salmoninae detection. The detection limits were 100 pg using five
species of Salmoninae as the templates. The relative detection limits were 0.01% (m/m) by mixing the fish of Salmo salar or
Salvelinus alpinus with maize, chicken or crucian. Standard deviations and relative standard deviations of Ct values for five
DNA concentrations were all in the acceptable range. Twenty-five commercial products labeled “containing salmon” were
tested by the real-time PCR assay, and four samples were detected without Salmoninae ingredient. Thus, considering its high
specificity, sensitivity and repeatability, we believed that the real-time PCR could be used to rapidly and accurately identify
Salmoninae ingredient in foods.

Key words: real-time polymerase chain reaction, Salmoninae, growth hormone gene, identification

李进波，李想，谌鸿超，潘良文，李正，宋青. 实时荧光PCR法鉴定食品中鲑亚科鱼成分[J]. 食品科学, doi: 10.7506/spkx1002-6630-201320041.

LI Jin-bo，LI Xiang，CHEN Hong-chao，PAN Liang-wen，LI Zheng，SONG Qing. Identification of Salmoninae in Foods by Real-Time PCR[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201320041.

[1]	项婷，夏琛，刘健华，王超然，沈建福. 蛹虫草中新化合物的分离纯化、结构鉴定及抗肿瘤活性[J]. 食品科学, 2021, 42(8): 235-242.
[2]	陈丽花，任丽霞，李东娜，马霞. 甜酒曲中优质酵母菌的分离鉴定及其产香特性分析[J]. 食品科学, 2021, 42(6): 142-149.
[3]	黄荣，王晓丽，王兆升，李游，方双杰. 皱皮木瓜腐败微生物的分离鉴定[J]. 食品科学, 2021, 42(4): 161-166.
[4]	弓敏，马艳秋，王瑞红，迟媛，迟玉杰. 鸡蛋壳表面典型菌株的分离鉴定及其对全蛋液致腐能力分析[J]. 食品科学, 2021, 42(4): 153-160.
[5]	孙悦，刘佳伊，陈璐，杜宏，白凤翎，吕欣然，张德福，郭晓华，励建荣. 抗耐药性大肠杆菌乳酸菌的筛选及抑菌机制[J]. 食品科学, 2021, 42(2): 121-127.
[6]	刘璐，吴江丽，杨金桃，唐忠月，曾雪峰. 发酵鱼酱酸产GABA乳酸菌的分离筛选及发酵特性[J]. 食品科学, 2021, 42(18): 73-79.
[7]	张娜娜，陈利容，张守梅，郭玉秋，刘开昌，龚魁杰. 鲜食玉米籽粒发育期间污染真菌分离鉴定及真菌毒素含量分析[J]. 食品科学, 2021, 42(16): 273-280.
[8]	邹谋勇，何理琴，孙启星，朱新贵. 产4-乙基愈创木酚酵母的鉴定及其在酱油中的应用[J]. 食品科学, 2021, 42(12): 138-144.
[9]	丁丽丽，吕欣然，高永悦，崔晓玲，王小咪，白凤翎，仪淑敏，郭晓华. 鱼肠道中抗氧化活性乳酸菌的筛选及鉴定[J]. 食品科学, 2021, 42(10): 127-132.
[10]	李祥，解玉丽，贾园园，张闪，王红艳，刘先吏，罗湘艳，唐存多. 基于基因组挖掘技术的新型谷氨酸脱羧酶基因挖掘及表达鉴定[J]. 食品科学, 2021, 42(10): 79-85.
[11]	刘立兵，李睿文，陈志敏，王金凤，孙晓霞，袁万哲，王建昌. 产气荚膜梭菌实时荧光PCR和实时荧光RPA检测方法的建立和比较[J]. 食品科学, 2020, 41(4): 268-272.
[12]	杜艳，陈复生，陈晨，刘营. 转基因检测中大豆蛋白DNA提取方法筛选及其lectin基因降解分析[J]. 食品科学, 2020, 41(4): 102-111.
[13]	陆洲，戴意强，Hafiz Abdul RASHEED，吴寒，夏秀东，董明盛. 植物乳杆菌D1501发酵黄浆水的抑菌活性及其中细菌素的分离与鉴定[J]. 食品科学, 2020, 41(24): 117-124.
[14]	黄钦钦，田亚平. 条斑紫菜蛋白酶解液α-葡萄糖苷酶和DPP-IV抑制活性的表征及肽成分解析[J]. 食品科学, 2020, 41(24): 110-116.
[15]	陈思睿，唐琳琳，冯建文，孙丽娜，王金玲. 高效降解柠檬酸酵母菌的筛选鉴定及其在红树莓果汁中降酸特性[J]. 食品科学, 2020, 41(22): 133-139.

实时荧光PCR法鉴定食品中鲑亚科鱼成分

Identification of Salmoninae in Foods by Real-Time PCR

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价