食品科学

• 分析检测 • 上一篇    下一篇

黄芪超微粉HPLC指纹图谱的建立

李 健,韩 林,马玉芳,胡新奇,黄一帆   

  1. 福建农林大学 中西兽医结合与动物保健福建省高校重点实验室,福建 福州 350002
  • 出版日期:2013-10-25 发布日期:2013-09-28

HPLC Fingerprint Analysis of Ultra-Micro Powder of Astragali Radix

LI Jian,HAN Lin,MA Yu-fang,HU Xin-qi,HUANG Yi-fan   

  1. University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Online:2013-10-25 Published:2013-09-28

摘要:

目的:建立黄芪超微粉高效液相色谱(HPLC)的指纹图谱,为其质量控制和科学评价提供依据。方法:采用Inertsil ODS-SP(4.6mm×250mm,5μm)色谱柱,甲醇-乙腈(1:1,V/V)为流动相A,超纯水为流动相B,进行梯度洗脱,流速0.8mL/min,柱温25℃,检测波长260nm。采用中药色谱指纹图谱相似度评价系统2004A进行相似度分析,并进行聚类分析。结果:建立了黄芪超微粉的指纹图谱,确定12个共有峰。聚类分析表明10批样品存在明显的南北地域差异。结论:此方法简便、准确、重复性好,可用于黄芪超微粉质量控制与评价。

关键词: 黄芪超微粉, 高效液相色谱法, 指纹图谱, 相似度

Abstract:

Objective: To establish an high performance liquid chromatography fingerprint for quality control and evaluation
of ultra-micro powder of Astragali Radix. Methods: Chromatographic separation was achieved on an Inertsil ODS-SP
column (4.6 mm × 250 mm, 5 μm) with mobile phase A (methanol-acetonitrile, 1:1) and mobile phase B (ultra-pure water)
at a flow rate of 0.8 mL/min. In addition, the detection wavelength and column temperature were set at 260 nm and 25 ℃,
respectively. Results: Twelve peaks common to the established fingerprints were identified. Cluster analysis suggested apparent
geographic differences between the samples from the north and the south. Conclusion: The proposed method is simple,
accurate, repeatable and suitable for the quality control and evaluation of Astragali Radix ultra-micro powder.

Key words: ultra-micro powder of Astragali Radix, high performance liquid chromatography (HPLC), chromatographic fingerprint, similarity