食品科学 ›› 2013, Vol. 34 ›› Issue (22): 10-14.doi: 10.7506/spkx1002-6630-201322003

• 工艺技术 • 上一篇    下一篇

羧甲基化大粒车前子多糖的制备及其生物活性研究

江乐明,樊灿梅,聂少平,周华璐,李建宇,谢明勇   

  1. 南昌大学 食品科学与技术国家重点实验室,江西 南昌 330047
  • 收稿日期:2013-06-29 修回日期:2013-10-29 出版日期:2013-11-25 发布日期:2013-12-05
  • 通讯作者: 谢明勇 E-mail:xmync@sina.com
  • 基金资助:

    国家自然科学基金地区项目;国家大学生创新训练项目

Preparation and Immunoregulatory Activity of Carboxymethyl Polysaccharide from the Seeds of Plantago asiatica L.

JIANG Le-ming,FAN Can-mei,NIE Shao-ping,ZHOU Hua-lu,LI Jian-yu,XIE Ming-yong   

  1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
  • Received:2013-06-29 Revised:2013-10-29 Online:2013-11-25 Published:2013-12-05
  • Supported by:

    ;National university student innovation test plan (NMOE)

摘要:

研究以氢氧化钠和一氯乙酸对大粒车前子多糖进行羧甲基化修饰的条件,同时采用体外树突状细胞模型评价了羧甲基化大粒车前子多糖的生物活性。在单因素试验基础上,通过正交试验进一步确定了最适修饰反应条件为NaOH溶液浓度4.5mol/L、一氯乙酸剂量2.835g、反应温度50℃。体外细胞实验研究表明:当羧甲基取代度在0.5~0.6范围内时,羧甲基化修饰能够显著增强大粒车前子多糖促进树突状细胞分泌细胞因子IL-12p70的功效。对大粒车前子多糖进行羧甲基修饰可提高其生物活性,为大粒车前子多糖羧甲基化衍生物的开发应用提供了理论依据。

关键词: 大粒车前子, 多糖, 羧甲基化修饰, 树突状细胞, 生物活性

Abstract:

Polysaccharide derived from the seeds of Plantago asiatica L. (PLP) was carboxymethylated in aqueous alkaline
medium of sodium hydroxide (NaOH) using monochloroacetic acid (MCA) as etherifying agent. The effects of reaction
parameters such as NaOH concentration, the amount of added MCA, temperature and reaction time were investigated using
single-factor and orthogonal array design. Temperature effect on the reaction was not significant. Caboxymethylated PLP
(CM-PLP) with the highest degree of substitution (DS) was obtained in 4.5 mol/L aqueous NaOH with the addition of 2.835 g
of MCA at 50 ℃. Meanwhile, CM-PLPs with high DS showed a significantly enhanced effect on IL-12p70 secretion by
dendritic cells (DCs). This study indicates a new approach to exploit the resource of PLP.

Key words: Plantago asiatica L., polysaccharide, carboxymethylation, dendritic cell, biological activity

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