食品科学 ›› 2013, Vol. 34 ›› Issue (22): 81-86.doi: 10.7506/spkx1002-6630-201322016

• 工艺技术 • 上一篇    下一篇

响应面法优化甜菊苷残渣中有效抗氧化物质提取工艺

洪怡蓝,徐文斌,任晓静,陈 洁,奚印慈   

  1. 上海海洋大学食品学院,上海 201306
  • 收稿日期:2013-06-29 修回日期:2013-10-28 出版日期:2013-11-25 发布日期:2013-12-05
  • 通讯作者: 徐文斌 E-mail:wbxu1201@msn.cn

Optimization of Extraction Conditions of Antioxidant Components from Stevia Residue after Stevioside Extraction by Response Surface Methdology by Response Surface Methodology

HONG Yi-lan,XU Wen-bin,REN Xiao-jin,CHEN Jie,XI Yin-ci   

  1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
  • Received:2013-06-29 Revised:2013-10-28 Online:2013-11-25 Published:2013-12-05

摘要:

以甜菊苷残渣为原料,在单因素试验的基础上,采用响应面试验设计,以DPPH自由基的清除能力和提取物中总酚得率为双响应值,对超声提取过程的影响因素即料液比、超声时间、超声功率,进行三因素三水平的试验设计,优化甜菊苷残渣中有效抗氧化物质的提取工艺。结果表明:甜菊苷残渣中有效抗氧化物质的提取工艺为料液比1:24.41(g/mL)、超声时间38.05min、超声功率164.62W,此时DPPH自由基清除率预测值为91.18%,总酚得率为16.49mg/g。结合实际操作,调整料液比1:24(g/mL)、超声时间38min、超声功率164W,并进行重复实验进行验证,平均DPPH自由基清除率为(90.47±1.43)%,总酚得率为(16.14±0.36)mg/g,接近预测值。

关键词: 甜菊苷残渣, 总酚, 超声提取, 响应面法

Abstract:

The ultrasonic extraction of antioxidant components from Stevia residue after stevioside extraction was studied
by single factor approach and response surface methodology. The extraction conditions were optimized with respect to
material/liquid ratio, ultrasonication duration and ultrasonic power using a three-variable, three-level experimental design.
In the optimization, DPPH radical scavenging capacity and the extraction yield of total polyphenols were used as response
variables. The optimal extraction conditions of material/liquid ratio, ultrasonication duration and ultrasonic power were
found to be 1:24.41 (g/mL), 38.05 min and 164.62 W, respectively. The predicted DPPH radical scavenging capacity and
yield of total polyphenol under the optimized conditions were 91.18% and 16.49 mg/g, respectively. From the view of practical
application, the modified extraction conditions were 1:24 (g/mL), 38 min and 164 W, under which repeated experiments
showed an average DPPH radical scavenging efficiency of (90.47 ± 1.43)% and an average yield of total polyphenols of
(16.14 ± 0.36) mg/g with good agreement with the predicted results.

Key words: Stevia residue after stevioside extraction, total polyphenols, ultrasonic extraction, response surface methodology

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