海蜇糖蛋白对小鼠脾脏细胞因子mRNA表达的影响

doi:10.7506/spkx1002-6630-201323061

食品科学 ›› 2013, Vol. 34 ›› Issue (23): 305-308.doi: 10.7506/spkx1002-6630-201323061

海蜇糖蛋白对小鼠脾脏细胞因子mRNA表达的影响

任国艳，邵征，曹力，曹利，张凡，郭金英，樊金玲，陈秀金，赵瑞红

河南科技大学食品与生物工程学院，河南洛阳 471003

收稿日期:2013-07-16 修回日期:2013-11-04 出版日期:2013-12-15 发布日期:2014-01-03
通讯作者: 任国艳 E-mail:guoyan.ren@yahoo.com.cn
基金资助:
国家自然科学基金项目(31000765)；河南科技大学青年基金资助项目(2010QN0001)

Effects of A Glycoprotein from Jellyfish (Rhopilema esculentum) on Cytokine mRNA Expression in BALB/c Mice

REN Guo-yan，SHAO Zheng，CAO Li，CAO Li，ZHANG Fan，GUO Jin-ying，FAN Jin-ling，CHEN Xiu-jin， ZHAO Rui-hong

College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471003, China

Received:2013-07-16 Revised:2013-11-04 Online:2013-12-15 Published:2014-01-03
Contact: Guo-Yan REN E-mail:guoyan.ren@yahoo.com.cn

摘要/Abstract

摘要：

研究海蜇糖蛋白(JGP-III)对BALB/c小鼠脾脏淋巴细胞细胞因子mRNA表达的影响，探讨JGP-Ⅲ对小鼠的免疫调节机理。选用54只BALB/c小鼠(18～22g，SPF级)，随机分为6组：模型对照组、正常对照组、阳性对照组(左旋咪唑25mg/(kg·d)，以体质量计)、低剂量组(JGP-Ⅲ 10mg/(kg·d))、中剂量组(JGP-Ⅲ 50mg/(kg·d))和高剂量组(JGP-Ⅲ 100mg/(kg·d))，每组9只。饲养21d后，无菌取脾脏，分离淋巴细胞，采用荧光定量RT-PCR法分析其细胞因子IL-2、IL-12、IFN-γ、IL-4、IL-6和IL-10 mRNA的相对表达量。结果显示：通过3个剂量组和3个对照组中细胞因子mRNA表达水平的对比，得出海蜇糖蛋白JGP-III能够促进细胞因子IL-2、IL-12、IFN-γ的mRNA表达水平，而抑制IL-4、IL-6和IL-10 mRNA的表达水平，且具有剂量依赖关系。进而推测JGP-Ⅲ可能是通过调节淋巴细胞Th1/Th2细胞因子mRNA的表达水平，来提高机体免疫能力。

关键词: 海蜇, 糖蛋白, 细胞因子, mRNA表达

Abstract:

In a previous study, we isolated a glycoprotein named JGP-III from jellyfish (Rhopilema esculentum). The specific
aim of the present study was to investigate the roles of JGP-III in the immunoregulatory mechanism in BALB/c mice by
investigating its effects on the mRNA expression of the Th1- and Th2-cytokines in the spleen lymphocytes of these mice. A total of
54 healthy BALB/c mice (SPF, 18－22 g) were randomly divided into 6 groups with 9 mice in each group: normal control, model
control, positive control, low dose (10 mg/(kg·d)), moderate dose (50 mg/(kg·d)) and high dose (100 mg/(kg·d)) groups, respectively.
After 21 days of these treatments, lymphocytes were prepared from the spleen of these mice and total RNA was
isolated from their lymphocytes. Fluorescent quantitative real-time polymerase chain reaction (FQ-RT-PCR) technique was
used to analyze the relative mRNA expression of Th1-cytokines (IL-2, IL-12 and IFN-γ) and Th2-cytokines (IL-4, IL-6 and
IL-10). Our results showed that JGP-Ⅲ promoted the mRNA expression levels of Th1-cytokines but inhibited the mRNA
expression levels of Th2-cytokines. Moreover, our results suggest that JGP-Ⅲ can improve the body’s immune function
probably by regulating the mRNA expression levels of Th1-/Th2-cytokines in spleen lymphocytes.

Key words: jellyfish, glycoprotein, cytokine, mRNA expression

中图分类号:

TS254.9

任国艳，邵征，曹力，曹利，张凡，郭金英，樊金玲，陈秀金，赵瑞红. 海蜇糖蛋白对小鼠脾脏细胞因子mRNA表达的影响[J]. 食品科学, 2013, 34(23): 305-308.

REN Guo-yan，SHAO Zheng，CAO Li，CAO Li，ZHANG Fan，GUO Jin-ying，FAN Jin-ling，CHEN Xiu-jin， ZHAO Rui-hong. Effects of A Glycoprotein from Jellyfish (Rhopilema esculentum) on Cytokine mRNA Expression in BALB/c Mice[J]. FOOD SCIENCE, 2013, 34(23): 305-308.

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海蜇糖蛋白对小鼠脾脏细胞因子mRNA表达的影响

Effects of A Glycoprotein from Jellyfish (Rhopilema esculentum) on Cytokine mRNA Expression in BALB/c Mice

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