RP-HPLC-ESI-MS/MS分离鉴定花生红衣原花青素A型和B型二聚体

doi:10.7506/spkx1002-6630-201323030

食品科学 ›› 2013, Vol. 34 ›› Issue (23): 142-146.doi: 10.7506/spkx1002-6630-201323030

RP-HPLC-ESI-MS/MS分离鉴定花生红衣原花青素A型和B型二聚体

陈洋，王冰，玉佳男，李宝丽，文云，刘睿*，谭军*

华中农业大学食品科学技术学院，环境食品学教育部重点实验室，湖北武汉 430070

收稿日期:2013-07-19 修回日期:2013-11-01 出版日期:2013-12-15 发布日期:2014-01-03
通讯作者: 刘睿 E-mail:liurui@mail.hzau.edu.cn
基金资助:
国家自然科学基金

Separation and Identification of A-type and B-type Dimers of Procyanidins from Peanut Skin by RP-HPLC-ESI-MS/MS

CHEN Yang，WANG Bing，YU Jia-nan，LI Bao-li，WEN Yun，LIU Rui*，TAN Jun*

Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology,
Huazhong Agricultural University, Wuhan 430070, China

Received:2013-07-19 Revised:2013-11-01 Online:2013-12-15 Published:2014-01-03
Contact: LIU Rui E-mail:liurui@mail.hzau.edu.cn

摘要/Abstract

摘要：

以花生红衣为原料，制备A型和B型原花青素低聚体。通过体积分数70%乙醇提取并采用AB-8大孔树脂纯化得到高纯度混合物-花生红衣原花青素(PSP)，反相高效液相色谱-质谱联用技术(RP-HPLC-MS/MS)对ToyopearlHW-40(S)凝胶色谱分离的两个级分PSP-3、PSP-5进行分离鉴定。结果表明：PSP的纯度大于98.0%，其得率为9.8%。PSP-3由2种B型原花青素二聚体组成，PSP-5由4种A型原花青素二聚体组成。

关键词: 花生红衣, A型原花青素, B型原花青素, 反相高效液相色谱-质谱联用(RP-HPLC-MS/MS)

Abstract:

A-Type and B-type oligomeric proanthocyanidins were prepared from the 70% ethanol extract of peanut skin
by purification on AB-8 macroporous adsorption resin and two fractions PSP-3 and PSP-5 were separated and identified
by reversed phase high-performance liquid chromatography-tandem mass spectrometry (RP-HPLC-MS/MS). Peanut skin
procyanidins (PSP) with a purity of over 98.0% and a recovery of 9.8% were obtained after the purification. PSP-3 was
composed of 2 B-type procyanidolic dimers and PSP-5 was composed of 4 A-type procyanidolic dimers.

Key words: peanut skin, A-type procyanidins, B-type procyanidins, reverse phase high performance liquid chromatography combined with tandem mass spectrometry (RP-HPLC-MS/MS)

中图分类号:

TS201.2

陈洋，王冰，玉佳男，李宝丽，文云，刘睿*，谭军*. RP-HPLC-ESI-MS/MS分离鉴定花生红衣原花青素A型和B型二聚体[J]. 食品科学, 2013, 34(23): 142-146.

CHEN Yang，WANG Bing，YU Jia-nan，LI Bao-li，WEN Yun，LIU Rui*，TAN Jun*. Separation and Identification of A-type and B-type Dimers of Procyanidins from Peanut Skin by RP-HPLC-ESI-MS/MS[J]. FOOD SCIENCE, 2013, 34(23): 142-146.

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RP-HPLC-ESI-MS/MS分离鉴定花生红衣原花青素A型和B型二聚体

Separation and Identification of A-type and B-type Dimers of Procyanidins from Peanut Skin by RP-HPLC-ESI-MS/MS

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