关键词: 超高效液相色谱-串联质谱(UPLC-MS/MS), 鱼, 孔雀石绿, 结晶紫, 残留

Abstract: Abstract：Purpose Malachite green (MG), leuco malachite green (LMG), crystal violet (CV)and leuco crystal violet (LCV) residues in fish were determinated simultaneously using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Methods Fish samples added with internal standard d5-MG, d6-LMG, d5-CV, d6-LCV were extracted in acetonitrile at first, and then degreased through neutral alumina column, dried in nitrogen, volumed by solution of acetonitrile distilled water (50:50, V/V) to UPLC-MS/MS analysis. Results The linearity was investigated over a concentration range of 1-50ng/mL for MG and LMG. The linearity was investigated over a concentration range of 1-100ng/mL for CV and LCV. The limit of detection of MG, LMG, CV and LCV are 0.05μg/kg and the limit of quantification of four analyses are 0.15μg/kg. Method recovery is made by addition of 50, 100, 200ng/mL standard solution, respectively. The results show that average recoveries are between 87.8% and 110.8%, and relative standard deviations (RSD, n=6) are lower than 4.32%. Conclusions This method is sensitive, accurate and reliable, which provide reference for multitude residues detection of aquatic fish products.

Key words: Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), Fish, Malachite green (MG), Crystal violet (CV), Residues