超高效液相色谱-同位素稀释质谱法测定配方奶粉中的泛酸

doi:10.7506/spkx1002-6630-201408042

食品科学 ›› 2014, Vol. 35 ›› Issue (8): 212-216.doi: 10.7506/spkx1002-6630-201408042

超高效液相色谱-同位素稀释质谱法测定配方奶粉中的泛酸

渠岩1，崔亚娟1，李全霞1，陈兆天1，李东1，梁敏慧2

1.北京市营养源研究所，北京 100050；2.北京工业大学生命科学与生物工程学院，北京 100022

收稿日期:2013-08-01 修回日期:2014-03-28 出版日期:2014-04-25 发布日期:2014-05-13
通讯作者: 渠岩 E-mail:quyan_0220@163.com
基金资助:
北京市科技新星计划项目（2011059）；卫生行业科研专项项目（201202012）；
北京市科委“双十计划”项目（Z121106002812109）；北京市优秀人才培养资助项目（2011D002022000001)

Determination of Pantothenic Acid in Formula Milk Powder Using Ultra Performance Liquid Chromatography-Isotope Dilution Mass Spectrometry

QU Yan1, CUI Ya-juan1, LI Quan-xia1, CHEN Zhao-tian1, LI Dong1, LIANG Min-hui2

1. Beijing Research Institute for Nutritional Resources, Beijing 100050, China;
2. College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100022, China

Received:2013-08-01 Revised:2014-03-28 Online:2014-04-25 Published:2014-05-13
Contact: QU Yan E-mail:quyan_0220@163.com

摘要/Abstract

摘要：

建立测定配方奶粉中泛酸的超高效液相色谱-同位素稀释质谱方法。样品经乙酸铵溶液提取、三氯甲烷除蛋白后，进行超高效液相色谱-串联质谱分析，采用HSS T3液相色谱柱分离，以10 mmol/L乙酸铵溶液（含0.1%甲酸）和乙腈为流动相进行梯度洗脱，多反应监测模式，内标法定量。结果表明：方法定量限为0.040 mg/100 g，线性范围内低、中、高3 个标准添加水平的回收率为96.9%～104.3%，相对标准偏差为3.78%～5.04%。该方法操作过程简单、分析周期短、灵敏度高、重复性好，适用于奶粉中泛酸的测定。

关键词: 泛酸, 超高效液相色谱-同位素稀释质谱方法, 配方奶粉

Abstract:

An ultra performance liquid chromatography-isotope dilution mass spectrometry (UPLC-IDMS) method has been
developed for the determination for pantothenic acid (PA) in formula milk powder. Samples were extracted with ammonium
acetate solution, and precipitated protein by adding chloroform for analyzing by UPLC-MS-MS. The analyte was separated
using an HSS T3 column. The mobile phase consisted of 10 mmol/L ammonium acetate with 0.1% formic acid and
acetonitrile. The PA was identified by multiple reaction monitoring (MRM) and quantified by internal standard method.
The results showed that the limit of quantification for PA was 0.040 mg/100 g. The recoveries at three spiked levels and the
relative standard deviations were 96.9%–104.3% and 3.78%–5.04%, respectively. This method proved to be simple, timesaving,
sensitive and accurate for the determination of PA in formula milk powder.

Key words: pantothenic acid, ultra performance liquid chromatography-isotope dilution mass spectrometry, formula milk powder

中图分类号:

渠岩1，崔亚娟1，李全霞1，陈兆天1，李东1，梁敏慧2. 超高效液相色谱-同位素稀释质谱法测定配方奶粉中的泛酸[J]. 食品科学, 2014, 35(8): 212-216.

QU Yan1, CUI Ya-juan1, LI Quan-xia1, CHEN Zhao-tian1, LI Dong1, LIANG Min-hui2. Determination of Pantothenic Acid in Formula Milk Powder Using Ultra Performance Liquid Chromatography-Isotope Dilution Mass Spectrometry[J]. FOOD SCIENCE, 2014, 35(8): 212-216.

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超高效液相色谱-同位素稀释质谱法测定配方奶粉中的泛酸

Determination of Pantothenic Acid in Formula Milk Powder Using Ultra Performance Liquid Chromatography-Isotope Dilution Mass Spectrometry

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