食品科学

• 工艺技术 • 上一篇    下一篇

双水相超声波法辅助提取甜玉米芯多酚及抑菌性研究

马永强,荆丽荣,刘晓飞,张 娜,刘晓凝   

  1. 哈尔滨商业大学 黑龙江省普通高等学校食品科学与工程重点实验室,黑龙江 哈尔滨 150076
  • 出版日期:2013-12-25 发布日期:2014-01-03

Ultrasonic-Assisted Aqueous Two-Phase Extraction and Antimicrobial Activity of Sweet Corncob Polyphenols

MA Yong-qiang,JING Li-rong,LIU Xiao-fei,ZHANG Na,LIU Xiao-ning   

  1. Key Laboratory for Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China
  • Online:2013-12-25 Published:2014-01-03

摘要:

通过单因素和正交试验,研究利用双水相超声波辅助提取甜玉米芯多酚类化合物的工艺。考察丙酮体积分数、硫酸铵用量、提取时间、料液比4个单因素对多酚提取率的影响。结果表明:通过正交试验得到多酚提取的最佳工艺为丙酮体积分数60%、硫酸铵用量0.175g/mL、提取时间25min、料液比1:20(g/mL),此条件下所得提取液的多酚提取率为4.41%。甜玉米芯多酚提取物有抑菌活性,对大肠杆菌、枯草芽孢杆菌、白葡萄球菌抑制作用明显。

关键词: 甜玉米芯, 多酚, 超声波提取, 抑菌活性

Abstract:

One-factor-at-a-time and orthogonal array designs were applied to establish for optimal conditions for ultrasonicassisted
aqueous two-phase extraction of polyphenols from sweet corncob. The effects of four process parameters including
acetone concentration, ammonium sulfate dosage, extraction time and solid-to-liquid ratio on the yield of polyphenols were
investigated. The optimal extraction conditions were found as follows: an aqueous two-phase system formed by adding 0.175 g/mL
of ammonium sulfate to 60% aqueous acetone, solid-to-solvent ratio of 1:20 (g/mL), and 25 min extraction. The yield of
polyphenols in extracts under these conditions was 4.41%. The polyphenols extracted from sweet corncob had significant
antibacterial activity against Escherichia coli, Bacillus subtilis and Staphylococcus white.

Key words: sweet corncob, polyphenols, ultrasonic-assisted extraction, antibacterial activity