反相C18柱层析法制备高纯度辣椒碱单体

doi:10.7506/spkx1002-6630-201324013

食品科学

反相C18柱层析法制备高纯度辣椒碱单体

王洋，韩爽，曹迪，刘举，周云鹏，徐利锋，陈烨

1.辽宁大学药学院，辽宁沈阳 110036，2.辽宁省肿瘤医院，辽宁沈阳 110042

出版日期:2013-12-25 发布日期:2014-01-03

Preparation of High Purity Capsaicin Monomer by Reverse Phase C18 Column Chromatography

WANG Yang，HAN Shuang，CAO Di，LIU Ju，ZHOU Yun-peng，XU Li-feng，CHEN Ye

1. College of Pharmacy, Liaoning University, Shenyang 110036, China；
2. Liaoning Cancer Hospital & Institute, Shenyang 110042, China

Online:2013-12-25 Published:2014-01-03

摘要/Abstract

摘要：

为得到纯品辣椒碱单体，去除辣椒碱类化合物其余成分的副作用，研究了高纯度辣椒碱单体制备方法。以辣椒精为原料，得到辣椒碱类化合物，利用多种柱色谱技术进行分离和纯化，最终用反相C18柱层析法得到高纯度辣椒碱单体。经高效液相色谱法检测辣椒碱类化合物为辣椒碱和二氢辣椒碱的混合物。辣椒碱单体纯度为98%。反相C18柱层析法能有效分离纯化辣椒碱类化合物，可以用于制备高纯度辣椒碱单体。

关键词: 辣椒碱单体, 辣椒碱类化合物, 反相C18柱层析

Abstract:

A novel method was developed to prepare high purity capsaicin monomer using reverse phase C18 column
chromatography. Capsaicinoids were extracted from capsicum oleoresin and then fractionated by column chromatographies
on silica gel, alumina and reverse phase C18 silica gel. As a result, a mixture was obtained consisting of capsaicin with
a purity of 98% as determined by HPLC and dihydrocapsaicin. This study suggests that reverse phase C18 column
chromatography is an effective method to purify capsaicinoids and prepare high-purity capsaicin monomer.

Key words: capsaicin monomer, capsaicinoids, reverse phase C18 column chromatography

王洋，韩爽，曹迪，刘举，周云鹏，徐利锋，陈烨. 反相C18柱层析法制备高纯度辣椒碱单体[J]. 食品科学, doi: 10.7506/spkx1002-6630-201324013.

WANG Yang，HAN Shuang，CAO Di，LIU Ju，ZHOU Yun-peng，XU Li-feng，CHEN Ye. Preparation of High Purity Capsaicin Monomer by Reverse Phase C18 Column Chromatography[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201324013.

反相C18柱层析法制备高纯度辣椒碱单体

Preparation of High Purity Capsaicin Monomer by Reverse Phase C18 Column Chromatography

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