食品科学 ›› 2014, Vol. 35 ›› Issue (1): 163-166.doi: 10.7506/spkx1002-6630-201401032

• 生物工程 • 上一篇    下一篇

PH值及底物对副干酪乳杆菌发酵生产苯乳酸的影响

王立梅1,2,腾 宇1,陈文飞1,张 辉1,储开阳1   

  1. 1.常熟理工学院生物与食品工程学院,江苏 常熟 215500;2.苏州市食品生物技术重点实验室,江苏 常熟 215500
  • 收稿日期:2013-07-27 修回日期:2013-12-23 出版日期:2014-01-15 发布日期:2014-01-22
  • 通讯作者: 王立梅 E-mail:wlmqb@126.com
  • 基金资助:

    国家农业科研成果转化资金项目(2013GB2C100176);江苏省科技支撑计划项目(BE2011393);
    苏州科技支撑计划项目(SN201130);常熟市科技发展计划项目(CN201220)

Effects of pH and Substrate Feeding on Phenyllactic Acid Production by Lactobacillus paracasei W2

WANG Li-mei1,2, TENG Yu1, CHEN Wen-fei1, ZHANG Hui1, CHU Kai-yang1   

  1. 1. School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China;
    2. Suzhou Key Laboratory of Food Biotechnlogy, Changshu 215500, China
  • Received:2013-07-27 Revised:2013-12-23 Online:2014-01-15 Published:2014-01-22
  • Contact: WANG Li-mei E-mail:wlmqb@126.com

摘要:

在7.5 L发酵罐中研究pH值及流加苯丙酮酸和葡萄糖对副干酪乳杆菌W2分批发酵产苯乳酸(phenyllacticacid,PLA)的影响。结果表明:发酵液初始pH值、底物葡萄糖和苯丙酮酸对菌体生长和产物产量都有影响。控制发酵液pH值为6.5,采用间歇流加苯丙酮酸及连续流加苯丙酮酸和葡萄糖,发酵36 h后PLA产量分别达到1.148 g/L和2.121 g/L,苯丙酮酸的转化率分别为62.19%和47.2%,与分批发酵相比分别提高41.72%和161.53%。

关键词: 副干酪乳杆菌, 苯乳酸, 苯丙酮酸, 底物流加

Abstract:

In this paper, the effects of initial medium pH and feeding of the substrates glucose and phenylpyruvic acid (PPA)
on the production of phenyllactic acid (PLA) by Lactobacillus paracasei W2 in a 7.5 L fermentor were studied. The results
indicated that all three factors generated corresponding effects on cell growth and PLA production. Two substrate feeding
strategies, intermittent feeding of PPA and continuous feeding of both glucose and PPA were performed and the initial
medium pH was set to 6.5. After 36 h of fermentation under these conditions, PLA production reached 1.148 g/L and 2.121 g/L,
respectively, and the conversion rate of PPA reached 62.19% and 47.2%, respectively. In addition, in comparison with fedbatch
fermentation, the PLA yield was enhanced by 41.72% and 161.53%, respectively.

Key words: Lactobacillus paracasei, phenyllactic acid, phenylpyruvic acid, substrate feeding

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