食品科学

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苦荞异槲皮苷对人胃癌细胞SGC-7901增殖及凋亡的影响

李玉英,赵淑娟,白崇智,张立伟,王转花   

  1. 1. 山西大学 化学生物学与分子工程教育部重点实验室,山西 太原 030006;
    2.山西省中医药研究院中心实验室,山西 太原 030012
  • 出版日期:2014-02-13 发布日期:2014-03-17

Effect of Isoquercetin from Fagopyrum tataricum on the Proliferation and Apoptosis of Human Gastric Carcinoma Cell Line SGC-7901

LI Yu-ying, ZHAO Shu-juan, BAI Chong-zhi, ZHANG Li-wei, WANG Zhuan-hua   

  1. 1. Key Laboratory for Chemical Biology and Molecular Engineering, Ministry of Education, Shanxi University, Taiyuan 030006, China;
    2. Central Laboratory of Shanxi Academy of Traditional Chinese Medicine, Taiyuan 030012, China
  • Online:2014-02-13 Published:2014-03-17

摘要:

从苦荞中提取制备异槲皮苷,研究其对人胃癌细胞SGC-7901增殖、凋亡、迁移和细胞周期的影响。将异槲皮苷作用于人胃癌SGC-7901细胞和人肾上皮细胞系293T,通过噻唑蓝法检测异槲皮苷对其增殖的影响;4’,6-二脒基-2-苯基吲哚(4’,6-diamino-2-phenyl indole,DAPI) 荧光染色法观察细胞核的形态学变化;划痕擦伤迁移实验检测异槲皮苷对SGC-7901细胞迁移能力的影响;流式细胞术检测异槲皮苷对SGC-7901细胞凋亡及细胞周期的影响。结果表明:苦荞异槲皮苷可以抑制SGC-7901细胞的增殖,并呈时间和剂量依赖性,当用100 μmol/L异槲皮苷作用细胞48 h后,对SGC-7901细胞的增殖抑制率达到35.92%, 而对人肾上皮细胞系293T的增殖抑制率仅为 3.15%;DAPI荧光染色法观察异槲皮苷处理细胞后,染色体凝聚,有凋亡小体产生;划痕擦伤实验显示,异槲皮苷能抑制SGC-7901细胞的迁移;流式细胞术检测结果表明,异槲皮苷可使G1和S期细胞减少,G2/M期细胞增多,且细胞凋亡率明显增加。综上所述,苦荞麦异槲皮苷能够诱导SGC-7901细胞发生凋亡,阻断细胞周期并抑制细胞增殖和迁移。

关键词: 异槲皮苷, 人胃癌细胞SGC-7901, 细胞周期, 凋亡, 细胞迁移

Abstract:

Isoquercetin was extracted from Fagopyrum tataricum and investigated for its effect on cell proliferation,
apoptosis and migration of human gastric carcinoma cell line SGC-7901. Cell proliferation was examined by using MTT
assay. The morphological changes of SGC-7901 were observed by DAPI nuclear staining. The effect of Fagopyrum
tataricum isoquercetin on cell migration was investigated by scratch-recovering tests in cell culture plates, as well as its
effect on cell apoptosis by flow cytometry. As a result, MTT assay showed that the isoquercetin could inhibit the viability
of SGC-7901 cells in time- and dose-dependent manners. SGC-7901 cell growth was inhibited by 35.92% or more at
isoquercetin concentration of 100 μmol/L for 48 h, compared to only 3.15% for 293T cells under the same conditions
of concentration and culture duration. DAPI nuclear staining showed chromosomal condensation and the production of
apoptotic bodies. Scratch-recovering tests indicated that the isoquercetin could suppress cell migration. Flow cytometry
showed that the number of cells at the G1 phase and S phase decreased and increased at the G2/M phase and the apoptotic cell
population exhibited a significant increase. Taken together, isoquercetin from F. tataricum can induce apoptosis, arrest cell
cycle and inhibit cell proliferation and migration of SGC-7901 cells.

Key words: isoquercetin, human gastric carcinoma cell line SGC-7901, cell cycle, apoptosis, cell migration