苦荞麸皮黄酮抗氧化及抗肿瘤活性

doi:10.7506/spkx1002-6630-201407012

食品科学

苦荞麸皮黄酮抗氧化及抗肿瘤活性

李富华，刘冬，明建

1.西南大学食品科学学院，重庆 400715；2.深圳职业技术学院应用化学与生物技术学院，广东深圳 518055；
3.西南大学国家食品科学与工程实验教学中心，重庆 400715；
4.农业部农产品贮藏保鲜质量安全与风险评估重点实验室，重庆 400715

出版日期:2014-04-15 发布日期:2014-04-18

Cellular Antioxidant and Antiproliferative Activities of Flavonoids Extracted from Tartary Buckwheat(Fagopyrum tartaricum (L.) Gaertn) Bran

LI Fu-hua, LIU Dong, MING Jian

1. College of Food Science, Southwest University, Chongqing 400715, China; 2. School of Applied Chemistry and Biological
Technology, Shenzhen Polytechnic, Shenzhen 518055, China; 3. National Food Science and Engineering Experimental Teaching
Center, Southwest University, Chongqing 400715, China; 4. Key Laboratory of Agricultural Products Quality Safety and Risk
Assessment during Storage, Ministry of Agriculture, Chongqing 400715, China

Online:2014-04-15 Published:2014-04-18

摘要/Abstract

摘要：

以西南地区的两种苦荞麸皮为实验材料（重庆酉阳苦荞麸皮（T1）；四川西昌苦荞麸皮（T2）），通过氧化自由基吸收能力实验（oxygen radical absorbance capacity，ORAC）测定苦荞麸皮黄酮的化学抗氧化能力，采用人肝癌细胞HepG2为细胞模型，研究苦荞麸皮黄酮的细胞抗氧化活性（cellular antioxidant activity，CAA）及对He pG2 细胞抗增殖活性。结果表明： T 1 和T 2 苦荞麸皮黄酮的ORAC值分别为（ 5 7 . 0±3 . 5 ）、（73.6±6.3）μmol TE/g。T1和T2苦荞麸皮黄酮的细胞抗氧化值分别为（44.4±5.2）、（52.5±2.7）μmolQE/100 g（PBS清洗）；（32.9±3.2）、（30.9±2.2）μmol QE/100 g（不经PBS清洗）。在对HepG2细胞体外抗增殖活性研究实验中，通过与对照组细胞相比，发现当苦荞麸皮黄酮的质量浓度为21.9 mg/mL时，T1和T2苦荞麸皮黄酮对HepG2细胞增殖的抑制率分别约为51%和82%（P＜0.01），二者相应的EC50值分别为（23.0±0.5）mg/mL和（13.7±0.1）mg/mL。因此，苦荞麸皮黄酮具有一定的体外细胞抗氧化和抗增殖的能力，可将苦荞麸皮作为此类功能性食品开发的原料资源。

关键词: 苦荞麸皮, 黄酮, 细胞抗氧化, 抗增殖, HepG2细胞

Abstract:

The oxygen radical absorbance capacity (ORAC) of flavonoids extracted from the bran of tartary buckwheat(Fagopyrum tartaricum (L.) Gaertn) from Youyang, Chongqing municipality (T1) and Xichang, Sichuan province (T2)was evaluated as well as cellular antioxidant (CAA) and antiproliferative activities on human liver cancer HepG2 cells.The results showed that the ORAC values of flavonoids extracted from T1 and T2 were (57.0 ± 3.5) and (73.6 ± 6.3) μmol TE/g,respectively, and the CAA values were (44.4 ± 5.2) and (52.5 ± 2.7) μmol QE/100 g by PBS washing; (32.9 ± 3.2) and(30.9 ± 2.2) μmol QE/100 g without PBS washing, respectively. The antiproliferative activity assay showed that at theconcentration of 21.9 mg/mL, the proliferation inhibitory rates of HepG2 cells by T1 and T2 flavonoids were approximately51% and 82%, respectively, when compared to control cells (P ＜ 0.01), and the corresponding EC50 (median effectiveconcentration) values were (23.0 ± 0.5) and (13.7 ± 0.1) mg/mL. Therefore, the flavonoids extracted from T1 and T2 hadsignificant CAA and anti-proliferative activities on human liver cancer HepG2 cells in vitro. This study shows that tartarybuckwheat bran can be considered as a resource of raw materials for functional foods.

Key words: Fagopyrum tartaricum (L.) Gaertn bran, flavonoids, cellular antioxidant activity, anti-proliferation, HepG2 cells

李富华，刘冬，明建. 苦荞麸皮黄酮抗氧化及抗肿瘤活性[J]. 食品科学, doi: 10.7506/spkx1002-6630-201407012.

LI Fu-hua, LIU Dong, MING Jian. Cellular Antioxidant and Antiproliferative Activities of Flavonoids Extracted from Tartary Buckwheat(Fagopyrum tartaricum (L.) Gaertn) Bran[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201407012.

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苦荞麸皮黄酮抗氧化及抗肿瘤活性

Cellular Antioxidant and Antiproliferative Activities of Flavonoids Extracted from Tartary Buckwheat(Fagopyrum tartaricum (L.) Gaertn) Bran

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