食品科学

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RS4型抗性淀粉对齐口裂腹鱼生长、脂肪代谢及相关基因表达的影响

向世琼,邬应龙*   

  1. 四川农业大学食品学院,四川 雅安 625014
  • 出版日期:2014-04-15 发布日期:2014-04-18

Effect of RS4-Type Resistant Starch on Growth Performance, Lipid Metabolism and Related Gene Expression in Schizothorax prenanti Tchang

XIANG Shi-qiong, WU Ying-long   

  1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
  • Online:2014-04-15 Published:2014-04-18

摘要:

目的:研究RS4型抗性淀粉对齐口裂腹鱼生长性能、脂肪代谢以及相关基因表达量的影响。方法:将体质量75 g左右的齐口裂腹鱼随机分为RS4型抗性淀粉添加量为0%(对照组)、3.5%、7%、14%、28% 5 组。饲喂60 d,测定生长性能指标、血脂水平,并采用实时荧光定量聚合酶链式反应技术检测各组肌肉及肝脏中过氧化物酶增殖体激活受体α(peroxisome proliferators activated receptors-alpha,PPARα)、脂肪酸结合蛋白(fatty acid-binding protein,FABP)mRNA表达水平。结果:添加RS4型抗性淀粉剂量为7%和14%时与对照组相比能显著增加齐口裂腹鱼的体质量、摄食率、特定生长率(P<0.05),添加剂量为28%时能够显著增加摄食率,但是对体质量和特定生长率没有显著的影响。添加剂量到达14%及以上时与对照组相比能够显著降低血脂水平及肌肉中心型脂肪酸结合蛋白(heart-fattyacid-binding protein,H-FABP mRNA)表达水平,且H-FABP与肌内脂肪呈正相关性。添加剂量达7%及以上时能够显著升高肌肉和肝脏中PPARα mRNA表达水平,降低肝脏中FABP mRNA表达水平。结论:适当的添加RS4型抗性淀粉能够促进齐口裂腹鱼的生长、并且调节脂质代谢相关基因的表达,降低了肠系脂肪及肌内脂肪沉积。

关键词: 抗性淀粉, 齐口裂腹鱼, 实时荧光定量聚合酶链式反应, 过氧化物酶增殖体激活受体&alpha, 脂肪酸结合蛋白

Abstract:

Objective: To explore the effect of RS4-type resistant starch on growth performance, lipid metabolism andrelated gene expression in Schizothorax prenanti Tchang. Methods: Schizothorax prenanti Tchang weighed about 75 g wererandomly divided into five groups which were fed RS4-type resistant starch at doses of 0 (control), 3.5%, 7%, 14% and 28%,respectively. After 60 days of administration, the growth performance and serum lipid levels were measured; meanwhile,peroxisome proliferators activated receptors-alpha (PPARα) and fatty acid-binding protein (FABP mRNA) expressionlevels in muscle and liver were determined by RT-PCR. Results: The body weight, feed intake and specific growth ratio ofSchizothorax prenanti Tchang were significantly higher in the 7% and the 14% resistant starch groups than in the controlgroup (P < 0.05). Feed intake was significantly increased at the dose of 28%, but neither body weight nor specific growthratio was significantly affected. The 14% resistant starch group showed significant reduced levels of blood lipids and heartfattyacid-binding protein (H-FABP) mRNA expression when compared to the control group, and a positive correlationexisted between H-FABP mRNA expression and intramuscular fat (IMF) content. When the dose was as high as 7% orabove, we observed a significant increase in PPARα mRNA expression levels in muscle and liver but a significant decreasein FABP mRNA expression level in liver. Conclusion: Dietary supplementation with the appropriate amount of RS4-typeresistant starch can promote the growth of Schizothorax prenanti Tchang, regulate lipid metabolism-related genes, andreduce the accumulation of mesenteric fat and intramuscular fat.

Key words: RS4-type resistant starch, Schizothorax prenanti Tchang, real time fluorescence quantitative polymerase chainreaction (RT-PCR), peroxisome proliferators activated receptors-alpha (PPARα), fatty acid-binding protein (FABP)