食品科学

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高光谱成像技术检测肴肉新鲜度

邹小波,李志华,石吉勇,黄晓玮   

  1. 江苏大学食品与生物工程学院,江苏 镇江 212013
  • 出版日期:2014-04-25 发布日期:2014-05-13
  • 基金资助:

    国家高技术研究发展计划(863计划)项目(2011AA100807);
    国家自然科学基金青年科学基金项目(61301239);江苏省杰出青年基金项目(BK20130010);
    新世纪优秀人才支持计划项目(NCET-11-0986);江苏省自然科学基金项目(BK20130505);
    江苏省普通高校研究生科研创新计划项目(CXZZ13_0697);中国博士后科学基金项目(2013M540422);
    江苏省博士后科研资助计划项目(1301051C);江苏大学高级专业人才科研启动基金项目(13JDG039)

Detection of Freshness Attributes of Yao Meat Based on Hyperspectral Imaging Technique

ZOU Xiao-bo, LI Zhi-hua, SHI Ji-yong, HUANG Xiao-wei   

  1. College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China
  • Online:2014-04-25 Published:2014-05-13

摘要:

建立阿胶中马和驴成分高特异、高灵敏的实时荧光聚合酶链式反应(polymerase chain reaction,PCR)检 测方法。选择马和驴线粒体基因tRNA-Thr及D-loop区为靶序列,设计合成特异引物,通过普通PCR和实时荧光PCR 检测,结果表明,这两对引物能够准确检测阿胶或动物胶中马和驴成分。

关键词: 高光谱成像技术, 肴肉, 新鲜度, 偏最小二乘

Abstract:

Total volatile basic nitrogen (TVB-N) content of Yao meat stored at 4 ℃ after the package was opened was
measured every 24 h for 8 days, and reflectance spectra were collected from the hyperspectral scattering images (HIS) in the
range of 430 to 960 nm. After pre-processed by standard normal variate transformation (SNV) method, prediction models
for TVB-N content in Yao meat were established by partial least squares (PLS) method, interval partial least squares (iPLS)
method, backward interval partial least squares (biPLS) method and synergy siPLS method separately. Experimental results
showed that the siPLS model could predict Yao meat TVB-N with correlation coefficient (Rp) of 0.854 8 and root mean
squared error of prediction (RMSEP) of 2.47, which was the best of the four models, and 430–461, 555–586 and 929–960
nm were the selected wavelength ranges. The overall prediction accuracy of the siPLS model for Yao meat freshness could
reach up to 87.5%. The present study shows that HIS technique is feasible for fast and non-destructive detection of Yao meat
freshness attributes.

Key words: hyperspectral imaging technique, Yao meat, freshness, partial least squares (PLS)

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