食品科学

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基于纳米金电化学免疫传感器测定牛奶中的青霉素G

李建龙,潘道东*,朱浩嘉,顾愿愿,赵紫微,朱珊珊   

  1. 宁波大学海洋学院,浙江 宁波 315211
  • 出版日期:2014-04-25 发布日期:2014-05-13
  • 通讯作者: 潘道东
  • 基金资助:

    “十二五”国家科技支撑计划项目(2012BAK08B01);宁波市创新团队项目(2012B82017);
    宁波市人事局人才基金项目(ZX2012000380)

Determination of Penicillin G in Milk by Gold Nanoparticles Based Electrochemical Immunosensor

LI Jian-long, PAN Dao-dong*, ZHU Hao-jia, GU Yuan-yuan, ZHAO Zi-wei, ZHU Shan-shan   

  1. School of Marine Sciences, Ningbo University, Ningbo 315211, China
  • Online:2014-04-25 Published:2014-05-13
  • Contact: PAN Dao-dong

摘要:

利用吸附法将青霉素G抗体固定于纳米金修饰的玻碳电极表面,制备用于检测青霉素G的电化学免疫传感 器,建立高度灵敏的一步直接电化学免疫法。纳米金的强吸附和导电作用,提高了青霉素G抗体的固定量和电化学 灵敏度。在优化条件下,该传感器的响应电流与青霉素质量浓度的对数在0.04~40.00 ng/mL范围内呈良好的线性关 系,相关系数为0.988 4,检测限为2.49 ng/mL,该法成功的实现了对牛奶中青霉素G的检测。

关键词: 纳米金, 青霉素, 电化学免疫传感器

Abstract:

An amperometric immunosensor was fabricated by co-immobilizing penicillin G polyclonal antibody (Ab*)
on the surface of a gold nanoparticle modified glassy carbon electrode through electrostatic adsorption. A highly sensitive
one-step direct electrochemical immunoassay was established due to the strong adsorption and conductive capacity of gold
nanoparticles, thus raising the amount of fixed penicillin G antibody and electrochemical sensitivity. Under optimized
conditions, the current response of the sensor showed a good linear relationship with logarithmic penicillin concentration in
the range of 0.04-40.00 ng/mL with a correlation coefficient of 0.988 4 and the detection limit was 2.49 ng/mL. The method
was able to detect penicillin in milk successfully.

Key words: gold nanoparticles, penicillin, electrochemical immunesensors

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