食品科学
• 分析检测 • 上一篇 下一篇
李建龙,潘道东*,朱浩嘉,顾愿愿,赵紫微,朱珊珊
出版日期:
发布日期:
通讯作者:
基金资助:
“十二五”国家科技支撑计划项目(2012BAK08B01);宁波市创新团队项目(2012B82017); 宁波市人事局人才基金项目(ZX2012000380)
LI Jian-long, PAN Dao-dong*, ZHU Hao-jia, GU Yuan-yuan, ZHAO Zi-wei, ZHU Shan-shan
Online:
Published:
Contact:
摘要:
利用吸附法将青霉素G抗体固定于纳米金修饰的玻碳电极表面,制备用于检测青霉素G的电化学免疫传感 器,建立高度灵敏的一步直接电化学免疫法。纳米金的强吸附和导电作用,提高了青霉素G抗体的固定量和电化学 灵敏度。在优化条件下,该传感器的响应电流与青霉素质量浓度的对数在0.04~40.00 ng/mL范围内呈良好的线性关 系,相关系数为0.988 4,检测限为2.49 ng/mL,该法成功的实现了对牛奶中青霉素G的检测。
关键词: 纳米金, 青霉素, 电化学免疫传感器
Abstract:
An amperometric immunosensor was fabricated by co-immobilizing penicillin G polyclonal antibody (Ab*) on the surface of a gold nanoparticle modified glassy carbon electrode through electrostatic adsorption. A highly sensitive one-step direct electrochemical immunoassay was established due to the strong adsorption and conductive capacity of gold nanoparticles, thus raising the amount of fixed penicillin G antibody and electrochemical sensitivity. Under optimized conditions, the current response of the sensor showed a good linear relationship with logarithmic penicillin concentration in the range of 0.04-40.00 ng/mL with a correlation coefficient of 0.988 4 and the detection limit was 2.49 ng/mL. The method was able to detect penicillin in milk successfully.
Key words: gold nanoparticles, penicillin, electrochemical immunesensors
中图分类号:
TS201.2
李建龙,潘道东*,朱浩嘉,顾愿愿,赵紫微,朱珊珊. 基于纳米金电化学免疫传感器测定牛奶中的青霉素G[J]. 食品科学, doi: 10.7506/spkx1002-6630-201408021.
LI Jian-long, PAN Dao-dong*, ZHU Hao-jia, GU Yuan-yuan, ZHAO Zi-wei, ZHU Shan-shan. Determination of Penicillin G in Milk by Gold Nanoparticles Based Electrochemical Immunosensor[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201408021.
0 / / 推荐
导出引用管理器 EndNote|Ris|BibTeX
链接本文: https://www.spkx.net.cn/CN/10.7506/spkx1002-6630-201408021
https://www.spkx.net.cn/CN/Y2014/V35/I8/111