食品科学

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傅里叶变换近红外光谱技术测定绿茶全氮量

杨 丹,刘 新*,张颖彬,尹 鹏   

  1. 中国农业科学院茶叶研究所,农业部茶叶质量监督检验测试中心,浙江 杭州 310008
  • 出版日期:2014-04-25 发布日期:2014-05-13
  • 通讯作者: 刘 新
  • 基金资助:

    国家现代农业(茶叶)产业技术体系建设专项(CARS-23)

Determination of Total Nitrogen in Green Tea by Fourier Transform Near Infrared Spectroscopy

YANG Dan, LIU Xin*, ZHANG Ying-bin, YIN Peng   

  1. Tea Quality and Supervision Testing Center, Ministry of Agriculture, Tea Research Institute of Chinese Academy of
    Agriculture Science, Hangzhou 310008, China
  • Online:2014-04-25 Published:2014-05-13
  • Contact: LIU Xin

摘要:

建立准确性高、稳定性好的绿茶全氮量的偏最小二乘定量分析模型。在4 691~3 959、5 126~4 848 cm-1 波段区间内,光谱经过一阶导数与平滑处理后,模型的预测性能最好,外部验证集样品的预测值与实测值的预测均 方差(RMSEP)为0.092 5%,相关系数为0.993 9。根据嫩度等级,建立了绿茶全氮量子模型,提高了模型的预测 性能。嫩度为3 级的子模型预测结果最好,验证集样品的全氮量的预测值与实测值的RMSEP值最小,为0.037 9%, 相关系数为0.996 1。绿茶全氮量近红外光谱检测技术是一种绿色、快速、高效的新型分析技术,能够实现快速测定 绿茶全氮量,评价绿茶品质。

关键词: 近红外光谱技术, 绿茶, 全氮量, 子模型

Abstract:

A highly accurate and stable calibration model of total nitrogen content in green tea was established based on
partial least square regression. The model demonstrated great predictive performance after the first derivative processing
and smoothing in the spectral range of 4 691–3 959 cm-1 and 5 126–4 848 cm-1. The correlation coefficient of predicted and
chemically measured values in the validation set was 0.993 9 and the RMSEP was 0.092 5%. The prediction performance
was improved by developing sub-models of total nitrogen in green tea according to different tenderness. The prediction
performance of the tenderness level 3 model was the best. The RMSEP in the validation set was the smallest, 0.037 9%,
and the correlation coefficient was 0.996 1. Thus, near infrared spectroscopy can provide a green, rapid and efficient
technique for the detection of total nitrogen in green tea and quality evaluation.

Key words: near infrared spectroscopy, green tea, total nitrogen content, sub-model

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