磁微粒酶联免疫吸附法测定玉米中的伏马毒素B1

doi:10.7506/spkx1002-6630-201408041

食品科学

磁微粒酶联免疫吸附法测定玉米中的伏马毒素B1

管笛1,2,3，潘灿平3，王文1,2，武会娟1,2,*

1.北京市理化分析测试中心，北京 100089；2.北京市食品安全分析测试工程技术研究中心，北京 100089；
3.中国农业大学理学院，北京 100094

出版日期:2014-04-25 发布日期:2014-05-13
通讯作者: 武会娟
基金资助:
北京市市级财政项目（PXM2013_178305_000002）

Development of a Magnetic Particle-Based Enzyme-Linked Immunosorbent Assay for Determining Fumonisin B1 in Corn

GUAN Di1,2,3, PAN Can-ping3, WANG Wen1,2, WU Hui-juan1,2,*

1. Beijing Centre for Physical and Chemical Analysis, Beijing 100089, China; 2. Beijing Engineering Research Center of Food
Safety Analysis, Beijing 100089, China; 3. College of Science, China Agricultural University, Beijing 100094, China

Online:2014-04-25 Published:2014-05-13
Contact: WU Hui-juan

摘要/Abstract

摘要：

Magnetic particles (MPs) were used as the support to replace enzyme-linked immunosorbent assay (ELISA) plates for
the determination of fumonisin B1 (FB1) in corn. Goat anti-rabbit antibody and protein A-MP conjugates were utilized in a direct
competitive ELISA format. Under optimized conditions, the limits of detection (LOD) for goat anti-rabbit antibody and protein
A-MPs were 0.024 and 0.030 μg/mL, respectively. The goat anti-rabbit antibody MP conjugates exhibited a lower LOD which
was only one third of that of conventional ELISA. The recoveries from spiked corn varied from 76.4% to 110.6%, with relative
standard deviation (RSD) less than 11%. Overall, a sensitive and stable method for FB1 has been developed in this paper.

关键词: magnetic particles, enzyme-linked immunosorbent assay, fumonisins, corn, protein A

Abstract:

Key words: magnetic particles, enzyme-linked immunosorbent assay, fumonisins, corn, protein A

中图分类号:

TS207.4

管笛1,2,3，潘灿平3，王文1,2，武会娟1,2,*. 磁微粒酶联免疫吸附法测定玉米中的伏马毒素B1[J]. 食品科学, doi: 10.7506/spkx1002-6630-201408041.

GUAN Di1,2,3, PAN Can-ping3, WANG Wen1,2, WU Hui-juan1,2,*. Development of a Magnetic Particle-Based Enzyme-Linked Immunosorbent Assay for Determining Fumonisin B1 in Corn[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201408041.

[1]	赵琳娜刘娜王学硕崔生辉. 肠道集聚性大肠埃希氏菌菌体和gDNA标准物质的研制[J]. 食品科学, 0, (): 0-0.
[2]	王丹丹李莉杨艳歌刘鸣畅王洪越吴淑清吴亚君. 克罗诺杆菌属快速检测体系的建立[J]. , 0, (): 0-0.
[3]	刘健慧张先舟张蕴哲李兰茹王红静高洁耿凤珍檀建新. 基于G-四链体的PCR-RCA双重扩增技术检测沙门氏菌[J]. 食品科学, 0, (): 0-0.
[4]	王玥沈明浩邵琳. 适配体结合量子点技术同时检测金黄色葡萄球和大肠埃希氏菌O157:H7方法[J]. , 0, (): 0-0.
[5]	王玥，邵琳，李乾学，易乐，曲晗，王洪利，沈明浩. 适配体结合量子点技术同时检测金黄色葡萄球菌和大肠埃希氏菌O157:H7方法[J]. 食品科学, 2021, 42(8): 305-312.
[6]	王晓瑞，邱红玲，王寿利，朱海华，周莉，平洋，谭静，王永，王慧. 抗副溶血弧菌OMPK单克隆抗体的制备及其ELISA双抗体夹心检测方法建立[J]. 食品科学, 2021, 42(4): 319-325.
[7]	闵可，张政，周雨蕾，侯温甫，王宏勋，周敏. 实时PCR检测食品中荧光假单胞菌方法的建立[J]. 食品科学, 2020, 41(24): 304-309.
[8]	刘希雅田春妹郑鹭飞钟平胜张馨方任佳丽. 磁性三维还原石墨烯的制备及其在电化学免疫检测牛乳中结核杆菌H37Ra的应用[J]. 食品科学, 0, (): 0-0.
[9]	刘希雅，田春妹，郑鹭飞，钟平胜，张馨方，任佳丽. 磁性三维还原石墨烯的制备及其在电化学免疫检测牛乳中结核杆菌H37Ra的应用[J]. 食品科学, 2020, 41(22): 308-314.
[10]	唐标罗怡李锐周秀楠张玲钱鸣蓉戴贤君夏效东杨华. 蜂花粉微生物污染及菌群结构分析[J]. , 0, (): 0-0.
[11]	张鹏飞，张杰，刘心雨，付雪婷，张萌，许学斌，吴聪明，姬华，王新. 上海市食源性耐甲氧西林金黄色葡萄球菌的分子特征及耐药性[J]. 食品科学, 2020, 41(20): 285-291.
[12]	唐标，罗怡，李锐，周秀楠，张玲，钱鸣蓉，戴贤君，夏效东，杨华. 蜂花粉微生物污染及菌群结构分析[J]. 食品科学, 2020, 41(20): 325-331.
[13]	夏丹丹，赵莹莹，马盼盼，王深垒，马常阳，郜晓峰，康文艺. 大肠杆菌DNA定性标准物质的制备及在枸杞子污染检测中的应用[J]. 食品科学, 2020, 41(18): 267-274.
[14]	苏晨丽陈兰明. 副溶血性弧菌毒素编码基因的微量、可视化检测[J]. 食品科学, 0, (): 0-0.
[15]	苏晨丽，陈兰明. 副溶血性弧菌毒素编码基因的微量、可视化检测[J]. 食品科学, 2020, 41(10): 288-296.

磁微粒酶联免疫吸附法测定玉米中的伏马毒素B1

Development of a Magnetic Particle-Based Enzyme-Linked Immunosorbent Assay for Determining Fumonisin B1 in Corn

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价