食品科学

• 技术应用 • 上一篇    

新霉素半定量胶体金试纸条的研制

王丽哲1,赵 瑜1,唐慧林1,王丽丽1,于重楠1,刘 敏1,何艳玲2   

  1. 1.北京陆桥技术有限责任公司,北京 100123;2.中国检验检疫科学研究院,北京 100123
  • 出版日期:2014-04-25 发布日期:2014-05-13
  • 基金资助:

    中国检验检疫科学研究院基本科研业务费专项资金项目(2012JK047)

Development of Semiquantitative Colloidal Gold Test Strip for Neomycin

WANG Li-zhe1, ZHAO Yu1, TANG Hui-lin1, WANG Li-li1, YU Zhong-nan1, LIU Min1, HE Yan-ling2   

  1. WANG Li-zhe1, ZHAO Yu1, TANG Hui-lin1, WANG Li-li1, YU Zhong-nan1, LIU Min1, HE Yan-ling2
  • Online:2014-04-25 Published:2014-05-13

摘要:

建立了一种快速、简单的检测牛乳中新霉素残留量的胶体金免疫层析法。将不同浓度的新霉素人工合成完 全抗原包被在硝酸纤维素膜上作为检测线(T1、T2线),与待测牛乳样品中的新霉素竞争结合新霉素单克隆抗体- 胶体金标记物,通过两条T1、T2线显色情况使传统新霉素胶体金试纸条由定性检测准确到半定量检测。试纸条对 牛乳样品的半定量检测限分别为200 μg/kg和400 μg/kg,该检测限满足欧盟及国家对新霉素检测的需求,且检测牛 乳样品能够满足现场快速检测的要求,为现场快速检测牛乳中新霉素残留提供了更精确的参考依据。

关键词: 新霉素, 半定量, 胶体金, 完全抗原, 现场检测, 快速检测

Abstract:

A simple and rapid method based on colloidal gold immunochromatography for the detection of
neomycin residues in milk was established. Two different concentrations of complete antigen for artificially synthetic
neomycin were coated onto a nitrocellulose membrane as the test lines (T1, T2), which could compete the binding between
neomycin monoclonal antibody coating colloidal gold and the free neomycin in milk samples. In this way, the accuracy of
traditional neomycin colloidal gold test strip was improved from qualitative to semi-quantitative detection by the color of
T1 and T2 lines. The semi-quantitative detection limits of the test lines T1 and T2 for milk samples were 200 and 400 μg/kg,
respectively, which could meet the requirement of the EU and the national standard for the determination of the maximum
residue limit (MRL) of neomycin. Also, this test strip enabled rapid on-site detection of neomycin residues in milk samples.

Key words: neomycin, semi-quantitative test, colloidal gold, complete antigen, field test, rapid detection

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